{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:15:17Z","timestamp":1790165717605,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2021,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>With an increasing aging population, postmenopausal osteoporosis has become a global public health problem. Previous evidence has shown that postmenopausal osteoporosis is a skeletal disease mainly caused by estrogen deficiency, generally accompanied by inflammation, and dietary isoflavones may ameliorate postmenopausal osteoporosis by anti\u2010inflammatory activity. We have generated isoflavone\u2010enriched soybean leaves (IESLs), but their anti\u2010inflammatory activity and effect on attenuating osteoporosis are still obscure. Here, we determined the isoflavone profiles of IESLs and evaluated their anti\u2010inflammatory activity in lipopolysaccharide\u2010stimulated RAW 264.7 cells and anti\u2010osteoporotic effects on ovariectomy\u2010induced osteoporosis in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>IESLs had a high content of total isoflavone. Hydrolysate of IESLs (HIESLs) was rich with the aglycones daidzein and genistein, and HIESLs can significantly inhibit lipopolysaccharide\u2010induced inflammation by reducing messenger RNA expression of <jats:italic>iNOS<\/jats:italic>, <jats:italic>COX\u20102<\/jats:italic>, <jats:italic>IL6<\/jats:italic>, and <jats:italic>IL1\u03b2<\/jats:italic>. Moreover, ovariectomized rats receiving aqueous extracts of IESLs (HIESLs) orally maintained more bone mass than control rats did, which was attributed to inhibition of osteoclastogenesis by downregulating the messenger RNA expression of the bone\u2010specific genes <jats:italic>RANKL\/OPG<\/jats:italic>, <jats:italic>OC<\/jats:italic>, and <jats:italic>cathepsin\u2009K<\/jats:italic>, and the inflammation\u2010related genes <jats:italic>IL6<\/jats:italic>, <jats:italic>NF\u03baB<\/jats:italic>, and <jats:italic>COX\u20102<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>IESLs may attenuate postmenopausal osteoporosis by suppressing osteoclastogenesis with anti\u2010inflammatory activity and be a potential source of functional food ingredients for the prevention of osteoporosis. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10763","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T14:37:18Z","timestamp":1599057438000},"page":"1499-1506","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Isoflavone\u2010enriched soybean leaves attenuate ovariectomy\u2010induced osteoporosis in rats by anti\u2010inflammatory activity"],"prefix":"10.1002","volume":"101","author":[{"given":"Cheng\u2010Liang","family":"Xie","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Science (Shenzhen) Sun Yat\u2010sen University  Guangzhou China"},{"name":"Department of Anatomy and Convergence Medical Science Institute of Health Sciences, College of Medicine, Gyeongsang National University  Jinju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki H","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Applied Life Science (BK21 plus) IALS, Gyeongsang National University  Jinju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang S","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Convergence Medical Science Institute of Health Sciences, College of Medicine, Gyeongsang National University  Jinju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kye M","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Food Science Gyeongnam National University of Science and Technology  Jinju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2588-1583","authenticated-orcid":false,"given":"Dong H","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Convergence Medical Science Institute of Health Sciences, College of Medicine, Gyeongsang National University  Jinju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10179"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11657-018-0534-5"},{"key":"e_1_2_6_4_1","first-page":"213","article-title":"Hormone replacement therapy and the prevention of postmenopausal osteoporosis","volume":"13","author":"Gambacciani M","year":"2014","journal-title":"Przegl Menopauzalny"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26187"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.08.014"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2016.231"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-018-0012-0"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b02543"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu8060361"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm7100297"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2018.02.014"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13765-013-3048-2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnim.2015.12.333"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-08046-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/108451"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17427-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201300390"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.10328"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.01.007"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2017.10.008"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-012-8331-5"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2016.08.007"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2013.01.001"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184904"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2017.03.004"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21020149"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29963-w"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.07.065"},{"key":"e_1_2_6_30_1","first-page":"1457","article-title":"Tanshinone IIA attenuates osteoclastogenesis in ovariectomized mice by inactivating NF\u2010\u03baB and Akt signaling pathways","volume":"10","author":"Cheng L","year":"2018","journal-title":"Am J Transl Res"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23071598"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2018.04.015"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.10763","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.10763","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.10763","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:34:33Z","timestamp":1760870073000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.10763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,11]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,3,15]]}},"alternative-id":["10.1002\/jsfa.10763"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.10763","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,11]]},"assertion":[{"value":"2020-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}