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During senescence, chlorophyll degradation is highly regulated in order to diminish toxicity of the free chlorophyll molecule due to its photoactivity. The first step in the chlorophyll degradation pathway is the conversion of chlorophyll b to chlorophyll a by means of two consecutive reactions catalyzed by enzymes coded by <jats:italic>NYC1<\/jats:italic> (<jats:italic>NON\u2010YELLOW COLORING 1<\/jats:italic>), <jats:italic>NOL<\/jats:italic> (<jats:italic>NYC1\u2010LIKE<\/jats:italic>) and <jats:italic>HCAR<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In this work, we studied the expression of <jats:italic>NOL<\/jats:italic> and <jats:italic>HCAR<\/jats:italic> genes during postharvest senescence of broccoli. We found that the expression of <jats:italic>BoNOL<\/jats:italic> increase during the first days of storage and then decrease. In the case of <jats:italic>BoHCAR<\/jats:italic>, its expression is maintained during the first days and then it also diminishes. Additionally, the effect of different postharvest treatments on the expression of these genes was also analyzed. It was observed that the expression of <jats:italic>BoNOL<\/jats:italic> is lower in the treatments performed with 1\u2010methylcyclopropene (1\u2010MCP), 6\u2010benzylaminopurine (6\u2010BAP) and modified atmospheres, while <jats:italic>BoHCAR<\/jats:italic> expression showed an increase in these same treatments, and a decrease in the treatment with ethylene. There were no variations in the expression of both genes in heat treatment, UV\u2010C treatment and visible light treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These results suggest that both <jats:italic>BoHCAR<\/jats:italic> and <jats:italic>BoNOL<\/jats:italic> show a lower regulation of their expression than other genes involved in chlorophyll degradation during senescence. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10783","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T05:46:20Z","timestamp":1600407980000},"page":"1629-1635","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Expression of <scp><i>BoNOL<\/i><\/scp> and <scp><i>BoHCAR<\/i><\/scp> genes during postharvest senescence of broccoli heads"],"prefix":"10.1002","volume":"101","author":[{"given":"Andrea M","family":"Reyes 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