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The objective of this study was to determine the effects of different doses of 25OHD<jats:sub>3<\/jats:sub> supplementation on performance, immunity, antioxidant capacity, intestinal morphology and bone quality in piglets.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>As dietary 25OHD<jats:sub>3<\/jats:sub> supplementation increased, the average daily gain (ADG) improved (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) quadratically during days 1\u201314, and tended to increase (<jats:italic>P<\/jats:italic> = 0.06) quadratically during the overall period of the experiment. Increasing 25OHD<jats:sub>3<\/jats:sub> supplementation increased (linear effect, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) the serum 25OHD<jats:sub>3<\/jats:sub> level and serum glutathione peroxidase (GSH\u2010Px) activity. On day 14, serum immunoglobulin A (IgA) was increased (linear and quadratic effects, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) as dietary 25OHD<jats:sub>3<\/jats:sub> supplementation increased. On day 28, serum IgA level was higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) linearly and the complement 3 (C3) level was reduced (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) linearly as dietary supplementation of 25OHD<jats:sub>3<\/jats:sub> increased. The mucosal GSH\u2010Px activity of the small intestine was higher (quadratic effect, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) with increasing 25OHD<jats:sub>3<\/jats:sub> supplementation. Jejunal villus height (<jats:italic>P<\/jats:italic> = 0.06) and villus height to crypt depth ratio (<jats:italic>P<\/jats:italic> = 0.07) tended to increase quadratically, and the villus height to crypt\u2010depth ratio of the ileum increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) linearly and quadratically with increasing 25OHD<jats:sub>3<\/jats:sub> supplementation. Dietary supplementation with an increasing level of 25OHD<jats:sub>3<\/jats:sub> increased breaking strength of tibias and femurs (quadratic effect, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Increasing dietary 25OHD<jats:sub>3<\/jats:sub> supplementation partly improved performance, immunity, antioxidant status, intestinal morphology, and bone properties of weaned piglets. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10889","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T04:03:43Z","timestamp":1602821023000},"page":"2592-2600","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Dietary 25\u2010hydroxycholecalciferol supplementation improves performance, immunity, antioxidant status, intestinal morphology, and bone quality in weaned piglets"],"prefix":"10.1002","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4604-452X","authenticated-orcid":false,"given":"Lianhua","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujie","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5913-8889","authenticated-orcid":false,"given":"Xiangshu","family":"Piao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition, College of Animal Science and Technology China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-012-9619-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-019-01020-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu5072502"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9060559"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2016.05.004"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007-0620"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2003.02377.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S000711451400097X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803217"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81809-E"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0271-5317(99)00141-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90590-H"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.111.031427"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2011-5023"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2009-00283"},{"key":"e_1_2_7_17_1","unstructured":"NRC Nutrient Requirements of Swine 11th edn. 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