{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T15:46:52Z","timestamp":1788968812166,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T00:00:00Z","timestamp":1605225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013211","name":"Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences","doi-asserted-by":"publisher","award":["1630032017036"],"award-info":[{"award-number":["1630032017036"]}],"id":[{"id":"10.13039\/501100013211","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Dietary intervention is an important approach to improve intestinal function of weaned piglets. Phytogenic and herbal products have received increasing attention as in\u2010feed antibiotic alternatives. This study investigated the chemical composition of guava leaf extract (GE) by ultrahigh\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2010MS\/MS). Meanwhile, we investigated the effects of dietary supplementation with GE on diarrhea in relation to immune responses and intestinal health in weaned piglets challenged by enterotoxigenic\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (ETEC).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In total, 323 characterized compounds, which including 91 phenolic compounds and 232 other compounds were identified. Animal experiment results showed that the supplementation of 50\u2013200 mg\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      of GE in the diet could reduce diarrhea incidence, increase activities of superoxide dismutase, glutathione peroxidase and total anti\u2010oxidant capacity in the serum (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), decrease the levels of interleukin 1\u03b2, interleukin 6 and tumor necrosis factor \u03b1 in the serum or jejunum mucosa (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), and increase villus height and villus height to crypt depth ratio (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) in the jejuna of piglets challenged by oral ETEC compared with negative control group (NC). Meanwhile, diet supplementation with 50\u2013200\u2009mg\u2009kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      GE reduced the levels of D\u2010lactate, endothelin\u20101 and diamine oxidase in the serum, and increased the expression of zonula occludens\u20101, Claudin\u20101, Occludin and Na\n                      <jats:sup>+<\/jats:sup>\n                      \/H\n                      <jats:sup>+<\/jats:sup>\n                      exchanger 3 (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05) in the jejuna mucosa of piglets challenged by ETEC compared with the NC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>These results suggested that GE could attenuate diarrhea and improve intestinal barrier function of piglets challenged by ETEC. \u00a9 2020 Society of Chemical Industry<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.10904","type":"journal-article","created":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T22:16:05Z","timestamp":1604355365000},"page":"2767-2778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Chemical composition and protective effect of guava (\n                    <i>Psidium guajava<\/i>\n                    <scp>L.<\/scp>\n                    ) leaf extract on piglet intestines"],"prefix":"10.1002","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4189-6213","authenticated-orcid":false,"given":"Dingfa","family":"Wang","sequence":"first","affiliation":[{"name":"Tropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Sciences  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Tropical Crops 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