{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T05:25:06Z","timestamp":1774761906669,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T00:00:00Z","timestamp":1637366400000},"content-version":"am","delay-in-days":356,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T00:00:00Z","timestamp":1606608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003382","name":"Core Research for Evolutional Science and Technology","doi-asserted-by":"publisher","award":["JPMJCR15O4"],"award-info":[{"award-number":["JPMJCR15O4"]}],"id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Insufficient intake of zinc is associated with various diseases worldwide. To overcome this problem, we aimed to establish a method for cultivating leafy vegetables with high zinc content in hydroponics without inhibiting their growth. Furthermore, we evaluated the effectiveness of the cultivated leafy vegetables with high zinc content in zinc\u2010deficient mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>By adjusting the zinc concentration in the hydroponic solution to 5\u00a0mg\u2009L<jats:sup>\u22121<\/jats:sup> starting from 7\u2009days before harvesting, the zinc content in leaf lettuce increased eight times of that in the control, without any inhibition of the growth. Furthermore, when oxidized glutathione (GSSG) was added simultaneously with zinc to the hydroponic solution, the zinc content further doubled (16 times of that in the control). Similar results were obtained with komatsuna and red leaf lettuce, although there was a difference in the effect of GSSG treatment. The effectiveness of leafy vegetables with high zinc content in ameliorating zinc deficiency was evaluated by feeding lettuce with high zinc content to zinc\u2010deficient mice. High zinc content lettuce significantly increased the zinc content in the liver, kidneys, gastrocnemius, and tibia of these mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>We established a cultivation method for lettuce and komatsuna with high zinc content without inhibiting growth by adjusting the zinc concentration in the hydroponic solution at an appropriate concentration for an appropriate period. The result of feeding test indicates that the intake of leafy vegetables with high zinc content can ameliorate zinc deficiency and might be useful in protection from several diseases associated with this deficiency. \u00a9 2020 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.10949","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T17:15:47Z","timestamp":1605892547000},"page":"3202-3207","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Establishment of a cultivation method for leaf lettuce (<scp><i>Lactuca sativa<\/i><\/scp> var. <i>crispa<\/i>) and komatsuna (<scp><i>Brassica rapa<\/i> var. <i>perviridis<\/i><\/scp>) with high zinc content for patients with zinc deficiency and evaluation of its effectiveness"],"prefix":"10.1002","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2537-7248","authenticated-orcid":false,"given":"Ogawa","family":"Atsushi","sequence":"first","affiliation":[{"name":"Department of Biological Production Akita Prefectural University  Akita Japan"},{"name":"Core Research for Evolutionary Science and Technology (CREST) Project Japan Science and Technology Agency  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kudo","family":"Ikumi","sequence":"additional","affiliation":[{"name":"Department of Biological Production Akita Prefectural University  Akita Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toyofuku","family":"Kyoko","sequence":"additional","affiliation":[{"name":"Department of Biological Production Akita Prefectural University  Akita Japan"},{"name":"Core Research for Evolutionary Science and Technology (CREST) Project Japan Science and Technology Agency  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,29]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"S99","article-title":"International Zinc Nutrition Consultative Group (IZiNCG) technical document# 1. Assessment of the risk of zinc deficiency in populations and options for its control","volume":"25","author":"Brown KH","year":"2004","journal-title":"Food Nutr Bull"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1079\/PNS2005474"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.1998.10718735"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2019.07.004"},{"key":"e_1_2_6_6_1","volume-title":"Vitamin and Mineral Requirements in Human Nutrition","author":"World Health Organization","year":"2004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3177\/jnsv.59.S91"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh064"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-017-1570-6_29"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04158.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/03650340.2017.1338343"},{"key":"e_1_2_6_12_1","first-page":"8","article-title":"Preparation of zinc\u2010enriched Kaiware radish sprouts and evaluation of their nutritional availability","volume":"25","author":"Yoshida M","year":"2014","journal-title":"Biomed Res Trace Elements"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2018.11.003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2018.10.018"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0098-8472(99)00013-1"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.11.1939"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/98.4.449"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/103.8.1175"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/118.6.699"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.10949","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.10949","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/jsfa.10949","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.10949","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:24:11Z","timestamp":1760865851000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.10949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,29]]},"references-count":18,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1002\/jsfa.10949"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.10949","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,29]]},"assertion":[{"value":"2020-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}