{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T11:03:48Z","timestamp":1785841428916,"version":"3.56.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T00:00:00Z","timestamp":1628553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31360381"],"award-info":[{"award-number":["31360381"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011328","name":"State Key Lab of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co. Ltd.","doi-asserted-by":"publisher","award":["K202003"],"award-info":[{"award-number":["K202003"]}],"id":[{"id":"10.13039\/501100011328","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Hypertension and diabetes are two kinds of senile diseases which often occur simultaneously. The commonly used drugs in clinic may produce certain side effects. Food\u2010derived polypeptide is a kind of polypeptide with great development potential, which has many functions of regulating human physiological function. Beer is rich in nutrition but there are few researches on bioactive peptides in beer.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In this study, a rapid virtual screening method was established to obtain bioactive peptides from Tsingtao draft beer. The peptide sequence was analyzed by ultra\u2010performance liquid chromatography\u2010quadrupole\u2010Orbitrap\u2010tandem mass spectrometry (UPLC\u2010Q\u2010Orbitrap\u2010MS<jats:sup>2<\/jats:sup>), and 50 peptides were identified. Eight peptides with potential biological activities were screened by using Peptide Ranker software and previous literature references. On the basis of absorption prediction, toxicity prediction, and molecular docking analysis, LNFDPNR and LPQQQAQFK were finally confirmed. The molecular docking results showed that two peptides could bind angiotensin\u2010converting enzyme (ACE) and dipeptidyl peptidase IV (DPP\u2010IV) tightly by hydrogen bonding and hydrophobic interaction. The <jats:italic>in vitro<\/jats:italic> activity evaluation results showed that two peptides had obvious ACE and DPP\u2010IV inhibitory activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study established a method for rapidly screening bioactive peptides from Tsingtao draft beer, screened two ACE and DPP\u2010IV inhibitory peptides in beer and analyzed their active action mechanism. This article may have great theoretical significance and practical value to further explore the health function of beer. \u00a9 2021 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.11445","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T09:57:21Z","timestamp":1627293441000},"page":"1085-1094","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Identification of <i>in vitro<\/i> angiotensin\u2010converting enzyme and dipeptidyl peptidase <scp>IV<\/scp> inhibitory peptides from draft beer by virtual screening and molecular docking"],"prefix":"10.1002","volume":"102","author":[{"given":"Tian","family":"Wenhui","sequence":"first","affiliation":[{"name":"State Key Laboratory of Biological Fermentation Engineering of Beer Tsingtao Brewery Co. Ltd. Qingdao  Qingdao China"},{"name":"Faculty of Agriculture and Food Kunming University of Science and Technology  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7889-9309","authenticated-orcid":false,"given":"Hu","family":"Shumin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biological Fermentation Engineering of Beer Tsingtao Brewery Co. Ltd. Qingdao  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7771-2610","authenticated-orcid":false,"given":"Zhuang","family":"Yongliang","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Food Kunming University of Science and Technology  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun","family":"Liping","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture and Food Kunming University of Science and Technology  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Hua","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biological Fermentation Engineering of Beer Tsingtao Brewery Co. Ltd. Qingdao  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2018.12.015"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3126\/ajms.v12i1.30508"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b02603"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2004.03.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b05618"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0797-673"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/157339909788166819"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.08.054"},{"key":"e_1_2_7_10_1","first-page":"1","article-title":"In vitro antioxidant and angiotensinz\u2010converting enzyme inhibitory activities of two oligopeptides in Jiupei of Baijiu","volume":"45","author":"Sun JY","year":"2019","journal-title":"Food Ferment Ind"},{"key":"e_1_2_7_11_1","first-page":"126","article-title":"Identification of a tripeptide Arg\u2010Asn\u2010His from Chinese Baijiu and its antioxidant activity","volume":"39","author":"Huo JY","year":"2018","journal-title":"Food Sci"},{"key":"e_1_2_7_12_1","first-page":"14","article-title":"Discovery of a bioactive peptide, an angiotensin converting enzyme inhibitor in Chinese Baijiu","volume":"16","author":"Wu JH","year":"2016","journal-title":"J Chin Inst Food Sci Technol"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2050-0416.2011.tb00467.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.07.038"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2050-0416.2003.tb00592.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jib.630"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio854"},{"key":"e_1_2_7_18_1","first-page":"126","article-title":"Identification and binding mechanism evaluation of novel ACE inhibitory peptides from ovalbumin","volume":"40","author":"Yu ZP","year":"2019","journal-title":"Food Science"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.14299"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1423"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0271-5317(01)00360-8"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.14975"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.03.061"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2017.06.008"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9101359"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2017.01.022"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.7b04196"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/53.370.947"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.07.111"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO02546K"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24173033"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.07.011"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-82502012000400019"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf051263l"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2020.109710"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-017-2879-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2014.07.005"},{"key":"e_1_2_7_38_1","first-page":"1","article-title":"Synthesis, molecular docking and ADMET prediction of novel swertiamarin analogues for the restoration of type\u20102 diabetes: an enzyme inhibition assay","author":"Kumar S","year":"2020","journal-title":"Nat Prod Res"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom9100569"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2014.04.018"},{"key":"e_1_2_7_41_1","first-page":"1","article-title":"Identification of potential dipeptidyl peptidase (DPP)\u2010IV inhibitors among Moringa oleifera phytochemicals by virtual screening, molecular docking analysis, ADME\/T\u2010based prediction, and in vitro analyses","volume":"25","author":"Yang Y","year":"2020","journal-title":"Molecules"},{"key":"e_1_2_7_42_1","first-page":"1","article-title":"Purification, characterization and molecular docking study of angiotensin\u2010i converting enzyme (ACE) inhibitory peptide from shortfin scad (Decapterus macrosoma) protein hydrolysate","author":"Ishak NH","year":"2021","journal-title":"J Food Sci Technol"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8FO00569A"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b00397"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2010.03.108"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b03956"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0FO01240H"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-019-09993-2"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-020-03553-5"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2016.03.005"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b01069"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.11445","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jsfa.11445","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.11445","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:08:40Z","timestamp":1760879320000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.11445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,10]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,2]]}},"alternative-id":["10.1002\/jsfa.11445"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.11445","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,10]]},"assertion":[{"value":"2021-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}