{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T14:29:39Z","timestamp":1784039379322,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>In this study, development and validation of a simple, miniaturized and, environmentally friendly carrier\u2010mediated three\u2010phase hollow\u2010fibre liquid\u2010phase micro\u2010extraction (HFLPME) technique was investigated for the enrichment of tetracycline residues in honey samples. The extracts were analysed using UV\u2013visible spectrophotometry and liquid chromatography\u2013quadrupole time\u2010of\u2010flight mass spectrometry (LC\u2010Q\u2010TOF\/MS). Parameters affecting the extraction efficiency of HFLPME such as pH of the donor and acceptor solutions, salt addition, agitation speed and extraction time were optimized.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The calibration curves showed good linearity, in the range of 1\u2013100\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup> with correlation coefficients ranging between 0.9943 and 0.9992, under the optimized conditions. Recoveries of blank honey samples at three spiking levels (1, 10 and 20\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup>) ranged from 81.2% to 107.5%. Relative standard deviations for the precision of the method were less than 15.0%. Limits of detection and limits of quantification were in the range of 0.0861\u20130.2628\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup> and 0.2610\u20130.7964\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup>, respectively. Finally, the proposed method was successfully applied in the extraction of five tetracyclines from honey samples. Doxycycline residue detected in one of the commercial honey samples was below the limit of quantification.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Because of the advantages offered by HFLPME, this method can be employed as an alternative to conventional extraction techniques for the clean\u2010up and pre\u2010concentration of antibiotics in complex matrices, including food samples. \u00a9 2021 Society of Chemical Industry<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.11663","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T03:09:58Z","timestamp":1637204998000},"page":"3204-3212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Enrichment of tetracycline residues from honey samples using carrier\u2010mediated hollow fibre liquid\u2010phase micro\u2010extraction and quantification by <scp>LC\u2010Q\u2010TOF<\/scp>\/<scp>MS<\/scp>"],"prefix":"10.1002","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1448-1146","authenticated-orcid":false,"given":"Babra","family":"Moyo","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Engineering and Agriculture University of Venda  Thohoyandou South Africa"},{"name":"Department of Food Science and Technology, Faculty of Science, Engineering and Agriculture University of Venda  Thohoyandou South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita T","family":"Tavengwa","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Engineering and Agriculture University of Venda  Thohoyandou South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.5772\/67135"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.01.012"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2018.1533477"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2015.12.013"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2939207"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2018.04.030"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cjoc.200790240"},{"key":"e_1_2_6_9_1","first-page":"293","article-title":"Validation of an analytical methodology for determination of tetracyclines residues in honey by UPLC\u2010MS\/MS detection","volume":"6","author":"Singh SP","year":"2015","journal-title":"Ind J Nat Prod Resour"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b01570"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2019.03.049"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2011.02.014"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2013.08.003"},{"key":"e_1_2_6_14_1","unstructured":"European Commission Regulation (EU) No 37\/2010 Off J Eur Union: L 15\/11\u201072(2010)."},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2018.11.015"},{"key":"e_1_2_6_16_1","first-page":"1538","article-title":"Development of a method based on DLLME and UFLC\u2010DAD for the determination of antibiotics in honey samples and the study of their degradation kinetics","volume":"29","author":"Santana AC","year":"2018","journal-title":"J Braz Chem Soc"},{"key":"e_1_2_6_17_1","unstructured":"GB 14963\u20132003 Ministry of Public Health of the People's Republic of China(2003)."},{"key":"e_1_2_6_18_1","unstructured":"Minist\u00e9rio da Agricultura Pecu\u00e1ria e Abastecimento (MAPA) Secretaria de Defesa Agropecu\u00e1ria (SDA); Instru\u00e7\u00e3o Normativa SDA No. 11 de 7 de maio de 2014;. 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