{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T14:32:48Z","timestamp":1779201168162,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Vitamin B<jats:sub>12<\/jats:sub> plays an important role in lipid, protein, carbohydrate and nucleic acid metabolism. We investigated the effect of supplementing layers\u2019 diets with different vitamin B<jats:sub>12<\/jats:sub> levels on liver metabolism using a liquid chromatography\u2013mass spectrometry\u2010based metabolomic approach to observe and analyse wide\u2010target metabolomics in the liver.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We assigned hens to three groups, namely blank control group without vitamin B<jats:sub>12<\/jats:sub> diet (BCG), normal control group with 25\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup> vitamin B<jats:sub>12<\/jats:sub> (NCG) and vitamin B<jats:sub>12<\/jats:sub> supplement group I with 100\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup> vitamin (VBSG I). The VBSG I group layers had higher (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) vitamin B<jats:sub>12<\/jats:sub> concentration than those from other groups. The egg yolk vitamin B<jats:sub>12<\/jats:sub> concentration increased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) with the increasing vitamin B<jats:sub>12<\/jats:sub> dietary supplemental level. Between the NCG <jats:italic>versus<\/jats:italic> BCG, VBSG I <jats:italic>versus<\/jats:italic> BCG, and VBSG I <jats:italic>versus<\/jats:italic> NCG groups, 11, 20 and 11 metabolites were significantly changed, respectively. The KEGG pathway of vitamin B<jats:sub>6<\/jats:sub> metabolism was significantly impacted in the NCG layers than those from BCG; seven and five pathways were significantly impacted in the VBSG I layers compared with those from BCG and NCG, including pyrimidine metabolism, vitamin B<jats:sub>6<\/jats:sub> metabolism, glycerophospholipid metabolism, etc.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>We concluded that 25\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup> vitamin B<jats:sub>12<\/jats:sub> supplementation in corn\u2013soybean meal\u2010based layer diet increased the egg yolk vitamin B<jats:sub>12<\/jats:sub> concentration and impacted the vitamin B<jats:sub>6<\/jats:sub> metabolic pathway, and 100\u2009\u03bcg\u2009kg<jats:sup>\u22121<\/jats:sup> of it increased the egg yolk and liver vitamin B<jats:sub>12<\/jats:sub> concentrations and impacted vitamin B<jats:sub>6<\/jats:sub>, lipid, nucleic acid and amino acid metabolic pathways. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.11928","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T21:39:38Z","timestamp":1649713178000},"page":"5787-5794","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of dietary supplementation of different levels of vitamin <scp>B<sub>12<\/sub><\/scp> on the liver metabolism of laying hens"],"prefix":"10.1002","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3480-9986","authenticated-orcid":false,"given":"Rui","family":"Wang","sequence":"first","affiliation":[{"name":"Laboratory of Poultry Production College of Animal Science, Shanxi Agricultural University  Jinzhong China"},{"name":"Department of Life Sciences Luliang University  Lvliang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Bai","sequence":"additional","affiliation":[{"name":"Laboratory 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