{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:41:32Z","timestamp":1768524092393,"version":"3.49.0"},"reference-count":43,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2022,7,11]],"date-time":"2022-07-11T00:00:00Z","timestamp":1657497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Caragana ambigua<\/jats:italic> has been the part of the dietary routines of the regional people in south\u2010west Pakistan and has traditionally been used for the treatment of diabetes there. There is an increased production of reactive oxygen species in diabetics, leading to gastrointestinal disorders. Natural antioxidants exhibit gastroprotective effects owing to their free\u2010radical scavenging action. <jats:italic>C.\u00a0ambigua<\/jats:italic> possesses appreciable phenolic and flavonoid content; thus, it has the potential to protect against gastrointestinal disorders (e.g. gastric ulcer).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>This study reports the anti\u2010ulcer potential of <jats:italic>C.\u00a0ambigua<\/jats:italic>. Four different fractions (chloroform, ethyl acetate, butanol, and aqueous) of plant were compared against omeprazole. Ulcer index, ulcer inhibition percentage, gastric pH and volume, total acidity, gastric protein, gastric wall mucus, and histopathology of gastric walls of rats were assessed. All fractions exhibited a reduction in ulcer index and promotion of percentage of ulcer inhibition compared with the ulcer control group. Furthermore, the fractions revealed a significant (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) diminution in gastric volume and total acidity with an increase in pH. Among the fractions investigated, the chloroform fraction unveiled the most promising anti\u2010ulcer activity, which is comparable to omeprazole. Liquid chromatography\u2013tandem mass spectrometry screening of fractions revealed the presence of formononetin and biochanin\u2009A (isoflavones reported to have anti\u2010ulcer properties) in the chloroform fraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study establishes that <jats:italic>C.\u00a0ambigua<\/jats:italic> possesses significant potential in reducing gastric ulcer progression. Formononetin and biochanin\u2009A are chiefly responsible for the stated bioactivity due to the fact that these compounds were solely present in the chloroform fraction. \u00a9 2022 The Authors. <jats:italic>Journal of The Science of Food and Agriculture<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12064","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T06:00:27Z","timestamp":1654927227000},"page":"7030-7038","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Gastroprotective effects of <i>Caragana ambigua<\/i> stocks on ethanol\u2010induced gastric ulcer in rats supported by <scp>LC\u2013MS<\/scp>\/<scp>MS<\/scp> characterization of formononetin and biochanin A"],"prefix":"10.1002","volume":"102","author":[{"given":"Faryal","family":"Rubab","sequence":"first","affiliation":[{"name":"Punjab University College of Pharmacy University of the Punjab  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hira","family":"Ijaz","sequence":"additional","affiliation":[{"name":"Punjab University College of Pharmacy University of the Punjab  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1712-6497","authenticated-orcid":false,"given":"Shah","family":"Hussain","sequence":"additional","affiliation":[{"name":"ADSI\u2013Austrian Drug Screening Institute GmbH  Innsbruck Austria"},{"name":"Department of Chemistry GC University  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ans","family":"Munir","sequence":"additional","affiliation":[{"name":"Punjab University College of Pharmacy University of the Punjab  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Stuppner","sequence":"additional","affiliation":[{"name":"ADSI\u2013Austrian Drug Screening Institute GmbH  Innsbruck Austria"},{"name":"Institute of Analytical Chemistry and Radiochemistry, CCB\u2010Center for Chemistry and Biomedicine Leopold\u2010Franzens University  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Jakschitz","sequence":"additional","affiliation":[{"name":"ADSI\u2013Austrian Drug Screening Institute GmbH  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcnther K","family":"Bonn","sequence":"additional","affiliation":[{"name":"ADSI\u2013Austrian Drug Screening Institute GmbH  Innsbruck Austria"},{"name":"Institute of Analytical Chemistry and Radiochemistry, CCB\u2010Center for Chemistry and Biomedicine Leopold\u2010Franzens University  Innsbruck 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