{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:49:55Z","timestamp":1791323395262,"version":"4.3.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>This study characterized an acidic polysaccharide (<jats:italic>OHC<\/jats:italic>\u2010LDPA) isolated from the medicinal and edible homologous plant <jats:italic>Onosma hookeri<\/jats:italic> Clarke var. <jats:italic>longiforum<\/jats:italic> Duthie. The structure of <jats:italic>OHC<\/jats:italic>\u2010LDPA was elucidated based on the analysis of infrared, one\u2011\/two\u2010dimensional nuclear magnetic resonance, and gas chromatography\u2013mass spectrometry data. The immunostimulatory effects of <jats:italic>OHC<\/jats:italic>\u2010LDPA were identified by both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> models.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The structure of <jats:italic>OHC<\/jats:italic>\u2010LDPA was elucidated as a typical pectin polysaccharide, consisting of galacturonic acid, galactose, arabinose, and rhamnose as the primary sugars, with linear galacturonic acid as the main chain and arabinogalacturonic acid as the main branched components. <jats:italic>OHC<\/jats:italic>\u2010LDPA could significantly stimulate the proliferation and phagocytosis of RAW264.7 macrophages and the release of nitric oxide <jats:italic>in vitro<\/jats:italic>. Also, it could accelerate the recovery of spleen and thymus indexes, enhance the splenic lymphocyte proliferation responses, and restore the levels of interleukin\u20102, interleukin\u201010, interferon\u2010<jats:italic>\u03b3<\/jats:italic>, and immunoglobulin\u2009G in the serum in a cyclophosphamide\u2010induced immunosuppressed\u2010mice model. In addition, <jats:italic>OHC<\/jats:italic>\u2010LDPA could restore the intestinal mucosal immunity and reduce the inflammatory damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:italic>OHC\u2010<\/jats:italic>LDPA could improve the immunity both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> and could be used as a potential immunostimulant agent. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12145","type":"journal-article","created":{"date-parts":[[2022,7,24]],"date-time":"2022-07-24T13:37:55Z","timestamp":1658669875000},"page":"328-338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Isolation, structure identification, and immunostimulatory effects <i>in vitro<\/i> and <i>in vivo<\/i> of polysaccharides from <i>Onosma hookeri<\/i> Clarke var. <i>longiforum<\/i> Duthie"],"prefix":"10.1002","volume":"103","author":[{"given":"Qiang","family":"Wu","sequence":"first","affiliation":[{"name":"Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University  Chengdu P. 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