{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:03:24Z","timestamp":1782817404697,"version":"3.54.5"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172279"],"award-info":[{"award-number":["32172279"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Exopolysaccharide biopolymers produced by microorganisms are crucial to the environment. They contribute to areas such as the health and bionanotechnology sectors, food and cosmetic industries as gelling agents, and environmental sector as flocculants owing to their biodegradability and non\u2010toxic nature. The current study aimed to isolate the fraction of released exopolysaccharide (rEPS) by <jats:italic>Bacillus velezensis<\/jats:italic> SN\u20101 from Chinese Da\u2010Jiang.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The weighted average molecular weight of the major isolated component, rEPS\u20102, was 202\u2009kDa, and its monosaccharide composition included mannose, glucose, and galactose at a molar ratio of 0.38:0.30:0.32. Further, the rEPS\u20102 was characterized using methylation analysis and one\u2010dimensional\/two\u2010dimensional nuclear magnetic resonance (1D\/2D NMR) spectroscopy. <jats:italic>In vivo<\/jats:italic> hepatoprotective effects indicated that rEPS\u20102 could alleviate carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)\u2010induced liver injury in mice by lowering the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and the malondialdehyde (MDA) levels. Furthermore, rEPS\u20102 can increase the expression of antioxidant genes HO\u20101, GCLC and NQO1 in the Nrf2\/ARE signaling pathway, thereby increasing the activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH\u2010Px) and reduced catalase (CAT) in liver cells. Furthermore, the rEPS\u20102 can be used and modulate the gut microbiota of mice with liver injury caused by CCl<jats:sub>4<\/jats:sub>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These results suggest that rEPS\u20102 has promising potential to serve as hepatoprotective agents. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12185","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T04:44:33Z","timestamp":1661316273000},"page":"738-749","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Structural characterization and hepatoprotective activity of exopolysaccharide from <i>Bacillus velezensis<\/i><scp>SN<\/scp>\u20101"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2830-9109","authenticated-orcid":false,"given":"Chengxu","family":"Cao","sequence":"first","affiliation":[{"name":"College of Food Science, Shenyang Agricultural University, Liaoning Engineering Research Centre of Food Fermentation Technology Shenyang Key Laboratory of Microbial Fermentation Technology Innovation  Shenyang P. 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