{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T15:35:11Z","timestamp":1791473711281,"version":"4.3.4"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Mulberry leaf extract (MLE) extracted from mulberry leaves is rich in a variety of bioactive ingredients and can be used as feed additives of weaned piglets. The present study was conducted to evaluate the effects of dietary MLE supplementation on intestinal barrier function, colon microbial numbers and microbial metabolites of weaned piglets.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>MLE supplementation increased the villus height and the villus height\/crypt depth ratio in jejunum and ileum (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), increased the mRNA expression of ZO\u20101, Claudin\u20101 and MUC\u20102 in the ileal mucosa (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and decreased the serum level of lipopolysaccharide (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). Meanwhile, MLE reduced the mRNA expression of tumor necrosis factor\u2010\u03b1 and interleukin\u20101\u03b2 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and increased secretory immunoglobulin A level in the ileal mucosa (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). In addition, MLE increased the numbers of beneficial bacteria <jats:italic>Bifidobacterium<\/jats:italic> and <jats:italic>Lactobacillus<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and decreased the number of potential pathogenic bacteria <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) in the colon. Correspondingly, MLE supplementation reduced the pH value of colonic digesta (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and altered the microbial fermentation pattern of the colon by increasing the concentrations of microbial metabolites derived from carbohydrates fermentation such as lactate, acetate, butyrate and total short\u2010chain fatty acids (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and decreasing the concentrations of microbial metabolites derived from amino acid fermentation such as <jats:italic>p<\/jats:italic>\u2010cresol, skatole, spermine, histamine and tryptamine (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>MLE supplementation improved intestinal barrier function and displayed beneficial effects on colon microbes and microbial metabolism in weaned piglets. \u00a9 2022 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12254","type":"journal-article","created":{"date-parts":[[2022,10,10]],"date-time":"2022-10-10T09:21:04Z","timestamp":1665393664000},"page":"1561-1568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Mulberry leaf extract improves intestinal barrier function and displays beneficial effects on colonic microbiota and microbial metabolism in weaned piglets"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4180-6399","authenticated-orcid":false,"given":"Min","family":"Song","sequence":"first","affiliation":[{"name":"Institute of Animal Science, Guangdong Academy of Agricultural Sciences, State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong 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