{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:07:57Z","timestamp":1790737677477,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T00:00:00Z","timestamp":1675382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006266","name":"Agencia de Desarrollo Econ\u00f3mico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009227","name":"Iowa State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009227","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,3,30]]},"abstract":"Abstract<\/jats:title>\n \n BACKGROUND<\/jats:title>\n \n Corn processing byproducts corn steep liquor (CSL), and thin stillage were evaluated as growth media for recombinant\n Lactococcus lactis<\/jats:italic>\n modified to produce antifreeze proteins (AFPs) that could have important food and non\u2010food applications. The AFP III sequence from ocean pout was cloned into a shuttle vector to make an expression vector that facilitated the production of recombinant AFP III in\n Lactococcus lactis<\/jats:italic>\n . Light CSL from yellow dent corn and thin stillage from the industrial corn bioethanol process were optimized as fermentation media with a combination of the following additives and trace elements: disodium\u2010\u03b2\u2010glycerophosphate (DG), tryptone (T), ascorbic acid (AA), iron (Fe), zinc (Zn), and magnesium (Mg). The growth of wild\u2010type and recombinant\n Lactococcus lactis<\/jats:italic>\n strains were compared over a 72\u2009h period in 96\u2010well plates and 250\u2009mL shake flasks.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n The corn coproducts media consisting of 50% (\n v\/v<\/jats:italic>\n ) light steep in water supplemented with DG\u20105\u00a0g\u2009L\n \u22121<\/jats:sup>\n , T\u20105\u00a0g\u2009L\n \u22121<\/jats:sup>\n , AA\u20100.5\u00a0g\u2009L\n \u22121<\/jats:sup>\n , and Zn\u20104\u00a0ppm resulted in best growth and was considered as the best\u2010optimized media. The addition of additives and trace elements better supported the growth of both wild\u2010type and recombinant\n Lactococcus lactis<\/jats:italic>\n strains compared to control media without any additives. Respective fermentation supernatants were frozen to \u221220\u2009\u00b0C, and the time to supercool and freeze was compared. A distinct supercooling effect was observed for the supernatants from recombinant strains thus, extending the time and temperature of supercooling and freezing. The maximum time of supercooling extended was 17.55\u2009\u00b1\u20094.45\u2009min for thin stillage followed by M17 media (17.25\u2009\u00b1\u20094.45\u2009min), Kent Corporation CSL (10.80\u2009\u00b1\u20092.12\u2009min), and yellow dent CSL (6.9\u2009\u00b1\u20090.85\u2009min) when fermented with recombinant\n Lactococcus lactis<\/jats:italic>\n strains.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n The supplemented corn coproduct\u2010based media enhanced the growth of both wild\u2010type and recombinant\n Lactococcus lactis<\/jats:italic>\n strains. These optimized media can replace or supplement more expensive media (e.g. M17), potentially reducing cost. The fermentation supernatants exhibited longer times to supercool, and freeze compared to control supernatants, indicating potential use as antifreeze compounds in frozen food and non\u2010food applications. \u00a9 2023 The Authors.\n Journal of The Science of Food and Agriculture<\/jats:italic>\n published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/jsfa.12461","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T09:19:56Z","timestamp":1674206396000},"page":"2512-2521","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Evaluation of corn steep liquor as fermentation media for recombinant\n Lactococcus lactis<\/i>\n producing antifreeze proteins"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-9912","authenticated-orcid":false,"given":"Bibek","family":"Byanju","sequence":"first","affiliation":[{"name":"Department of Food Science and Human Nutrition Iowa State University Ames IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swastik","family":"Sen","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Iowa State University Ames IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Mansell","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering Iowa State University Ames IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7774-3406","authenticated-orcid":false,"given":"Buddhi P.","family":"Lamsal","sequence":"additional","affiliation":[{"name":"Department of Food Science and Human Nutrition Iowa State University Ames IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1385\/ABAB:128:1:047"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-011-0959-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/en11071740"},{"key":"e_1_2_6_5_1","first-page":"203","article-title":"Production of penicillin in corn steep media with continuous glucose, sucrose, and lactose addition","volume":"6","author":"Ngerebara NN","year":"2017","journal-title":"J Environ Sci Comput Sci Eng Technol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.69170"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119460381.ch8"},{"key":"e_1_2_6_8_1","first-page":"293","article-title":"Pilot\u2010scale lactic acid production via batch culturing of Lactobacillus sp. 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