{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:24:53Z","timestamp":1781011493936,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The intestinal epithelium acts as a barrier against harmful luminal materials, thus preventing intestinal diseases and maintaining intestinal health. Heat shock protein 27 (HSP27) promotes intestinal epithelial integrity under both physiological and stressed conditions. The effects of partially hydrolyzed guar gum (PHGG) on HSP27 expression in intestinal Caco\u20102 cells and mouse intestines were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The present study showed that PHGG upregulated HSP27 expression in Caco\u20102 cells without upregulating <jats:italic>Hspb1<\/jats:italic>, the gene encoding HSP27. Feeding PHGG increased HSP25 expression in epithelial cells of the small intestine of mice. Inhibition of protein translation using cycloheximide suppressed PHGG\u2010mediated HSP27 expression, indicating that PHGG upregulated HSP27 via translational modulation. Signaling inhibition of the mechanistic target of rapamycin (mTOR) and phosphatidyl 3\u2010inositol kinase reduced PHGG\u2010mediated HSP27 expression, whereas mitogen\u2010activated protein kinase kinase inhibition by U0126 increased HSP27 expression, irrespective of PHGG administration. PHGG increases mTOR phosphorylation and reduces extracellular signal\u2010regulated protein kinase (ERK) phosphorylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>PHGG\u2010mediated translation of HSP27 in intestinal Caco\u20102 cells and mouse intestine via the mTOR and ERK signaling pathways may promote intestinal epithelial integrity. These findings help us better understand how dietary fibers regulate the physiological function of the intestines. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12551","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T22:33:19Z","timestamp":1678746799000},"page":"5165-5170","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Partially hydrolyzed guar gum upregulates heat shock protein 27 in intestinal <scp>Caco\u20102<\/scp> cells and mouse intestine <i>via<\/i><scp>mTOR<\/scp> and <scp>ERK<\/scp> signaling"],"prefix":"10.1002","volume":"103","author":[{"given":"Dina Mustika","family":"Rini","sequence":"first","affiliation":[{"name":"Graduate School of Integrated Sciences for Life Hiroshima University  Higashihiroshima Hiroshima Japan"},{"name":"Department of Food Technology, Faculty of Engineering Universitas Pembangunan Nasional 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