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Probiotics have been found to relieve constipation, which solves a health problem that constantly troubles a lot of people. Therefore, the changes in the metabolites of fermented yogurt with or without 10% defatted hempseed meal (10% SHY or 0% SHY respectively) were studied and their laxative effects were examined through animal experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Amino acids and peptides, terpene glycosides, carbohydrates, lineolic acids, and fatty acids were found to be the major contributors to the discrimination of the metabolic profile between 0% SHY and 10% SHY. The differentially accumulated metabolites may lead to the discrepancy in the yogurt's functionality. Animal experiments showed that the 10% SHY treatment prevented constipation by increasing feces number, fecal water content, and small intestinal transit rate and reducing inflammatory injury in loperamide\u2010induced constipated rats. Further analysis of the gut microbiota revealed that 10% SHY gavage increased the relative abundances of the <jats:italic>Lactobacillus<\/jats:italic>, <jats:italic>Allobaculum<\/jats:italic>, <jats:italic>Turicibacter<\/jats:italic>, <jats:italic>Oscillibacter<\/jats:italic>, <jats:italic>Ruminococcus<\/jats:italic>, and <jats:italic>Phascolarctobacterium<\/jats:italic> genera in the constipated rats, whereas <jats:italic>Akkermansia<\/jats:italic>, <jats:italic>Clostridium<\/jats:italic>_XIVa, <jats:italic>Bacteroides<\/jats:italic>, <jats:italic>Staphylococcus<\/jats:italic>, and <jats:italic>Clostridium<\/jats:italic>_IV were decreased. The combination of defatted hempseed meal and probiotics was found to be effective in relieving constipation, probably due to the enriched amino acids and peptides, such as Thr\u2010Leu and lysinoalanine through correlation analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Our findings indicated that defatted hempseed meal in yogurt altered the metabolic profile and effectively alleviated constipation in rats, which is a promising therapeutic candidate for constipation. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12575","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T09:04:18Z","timestamp":1679907858000},"page":"4778-4791","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Defatted hempseed meal altered the metabolic profile of fermented yogurt and enhanced the ability to alleviate constipation in rats"],"prefix":"10.1002","volume":"103","author":[{"given":"Jiaxin","family":"Xu","sequence":"first","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Hua","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Song","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1619-6330","authenticated-orcid":false,"given":"Lina","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Bohai University  Jinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jifeng","family":"Li","sequence":"additional","affiliation":[{"name":"Liaoning Qiaopai Biotech Co. 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