{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T23:14:46Z","timestamp":1773616486626,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Overuse of antibiotics contributes to bacterial resistance in animals. Therefore, it is necessary to find a new way to ensure animal health and promote animal growth. This experiment was conducted to investigate the effect of mannan oligosaccharide (MOS)\/vitamin E (VE)\/attapulgite (APT) nanocomposites (SLK1, SLK3, SLK5) on growth performance and intestinal health in weaned piglets. Each 1\u2009kg of SLK1, SLK3 or SLK5 contains 50\u2009g of vitamin E, and each had a different MOS concentration [SLK1 (50\u2009g\u2009kg <jats:sup>\u22121<\/jats:sup> MOS), SLK3 (100\u2009g\u2009kg <jats:sup>\u22121<\/jats:sup> MOS), SLK5 (150\u2009g\u2009kg <jats:sup>\u22121<\/jats:sup> MOS)]. In total, 135 piglets were randomly divided into five groups (normal control group, traditional antibiotic substitutes group, SLK1 group, SLK3 group and SLK5 group), and growth performance, diarrhea index, intestinal epithelial barrier function and intestinal microbial composition were measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>SLK1 and SLK5 significantly decreased diarrhea frequency in weaned piglets (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Furthermore, SLK5 significantly increased survival rate of weaned piglets compared to the traditional antibiotic substitutes group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). SLK5 also increased villus height of ileum, and increased goblet number of the jejunum (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). 16S rRNA sequencing showed that SLK5 significantly regulated intestinal colonic microbiota composition (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Specifically, SLK5 significantly increased the abundance of <jats:italic>Phascolarctobacterium succinatutens<\/jats:italic> in the cecum and increased the abundance of <jats:italic>Lactobacillus<\/jats:italic> and <jats:italic>Bifidobacterium<\/jats:italic> in the colon (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05)<jats:italic>.<\/jats:italic> In addition, dietary supplementation with 1\u2009kg\u2009T<jats:sup>\u22121<\/jats:sup> SLK5 also significantly increased the propionate content in the colon, which is significantly correlated with <jats:italic>Phascolarctobacterium<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05)<jats:italic>.<\/jats:italic><\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Dietary supplementation with 1\u2009kg\u2009T<jats:sup>\u22121<\/jats:sup> SLK5 improved intestinal epithelial barrier function, and regulated intestinal microbiota composition to prevent diarrhea in weaned piglets. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12642","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T14:54:39Z","timestamp":1681829679000},"page":"5569-5577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The nanocomposites of modified attapulgite with vitamin E and mannan oligosaccharide regulated the intestinal epithelial barrier and improved intestinal microbiota composition to prevent diarrhea in weaned piglets"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0319-7630","authenticated-orcid":false,"given":"Andong","family":"Zha","sequence":"first","affiliation":[{"name":"Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro\u2010Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture, Chinese Academy of Sciences  Changsha China"},{"name":"Institute of Subtropical Agriculture University of Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bie","family":"Tan","sequence":"additional","affiliation":[{"name":"Animal Nutrition Department College of Animal Science and Technology, Hunan Agricultural University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Nutrition Department College of Animal Science and Technology, Hunan Agricultural University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Qi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro\u2010Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture, Chinese Academy of Sciences  Changsha China"},{"name":"Institute of Subtropical Agriculture University of Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuankun","family":"Deng","sequence":"additional","affiliation":[{"name":"Animal Nutrition Department College of Animal Science and Technology, Hunan Agricultural University  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5740-7272","authenticated-orcid":false,"given":"Peng","family":"Liao","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro\u2010Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture, Chinese Academy of Sciences  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulong","family":"Yin","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro\u2010Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical Agriculture, Chinese Academy of Sciences  Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp911516b"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2141\/jpsa.0160071"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13041"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-021-00661-4"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2020.116685"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101640"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.08.107"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.2c00482"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps\/pez070"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.12.093"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201900521"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.8.3391-3395.2001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/03079450120106000"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skac184"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.11.006"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/5363546"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.828165"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-016-0063-2"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2014.14241"},{"key":"e_1_2_9_21_1","doi-asserted-by":"crossref","unstructured":"Bolyen E Rideout JR Dillon MR Bokulich NA Abnet C Al\u2010Ghalith GA et al. Reproducible interactive scalable and extensive microbiome data science using QIIME 2. Nat Biotechnol.37:852\u2013857(2019).https:\/\/doi.org\/10.1038\/s41587-019-0209-9.","DOI":"10.1038\/s41587-019-0209-9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-018-1335-9"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10110482"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00685-5"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/spsu.2001.22383"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-8140(04)80038-0"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029314"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.32"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.05.004"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2021.05.001"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00206-19"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/82_2012_217"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-070119-115104"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.SGE_Macfarlane"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(82)80339-9"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1159\/000492853"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/advances\/nmx009"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-017-0452-3"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.07.014"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2011-3921"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.12642","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:37:54Z","timestamp":1760877474000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.12642"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,12]]},"references-count":39,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,8,30]]}},"alternative-id":["10.1002\/jsfa.12642"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.12642","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,12]]},"assertion":[{"value":"2022-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}