{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T16:09:51Z","timestamp":1780157391886,"version":"3.54.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:00:00Z","timestamp":1683849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The health of elderly individuals is closely linked to their protein intake and the abundance of intestinal microbiota. To investigate the impact of a compound powder made from mulberry leaf and konjac (hereinafter referred to as \u2018compound powder\u2019) on regulating the structure of intestinal microbiota in 15\u2010month\u2010old BALB\/c mice that were fed a high\u2010beef\u2010protein diet, 16S rRNA high\u2010throughput sequencing, reverse transcription quantitative polymerase reaction, western blot, and other biochemical methods were used to analyze the differences in intestinal microbiota, protein metabolism\u2010related genes, short\u2010chain fatty acid (SCFA) content, and serum cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The results showed that the compound powder increased the content of SCFAs, reduced the inflammatory reaction of the body, adjusted the abundance of intestinal microbiota (Firmicutes and Bacteroidetes), and increased the ratio of Firmicutes to Bacteroidetes (F\/B). Moreover, the compound powder could increase the abundance of <jats:italic>Lactobacillus<\/jats:italic> and some non\u2010dominant bacteria that were related to amino acid metabolism and beneficial to human health, such as <jats:italic>Eubacterium coprostanoligenes<\/jats:italic>. These beneficial bacteria competitively reduced the abundance of harmful bacteria to protect the intestinal barrier and promote intestinal health, and upregulated the activities of aminopeptidase, proton\u2010coupled oligopeptide transporter 1, and glutamate dehydrogenase at the transcription and translation levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The compound powder could balance the abundance of intestinal microbiota, which may improve the metabolic capacity of old mice on a high\u2010protein diet, and ultimately promoting the well\u2010being of elderly individuals. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12664","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:34:35Z","timestamp":1682987675000},"page":"5949-5957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mulberry leaf and konjac 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