{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:07:11Z","timestamp":1791986831540,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Mycoplasma hyorhinis<\/jats:italic> is a prevalent respiratory pathogen in swine, causing significant economic loss to pig producers. There is growing evidence that respiratory pathogen infections have a large impact on intestinal microecology. To study the effect of <jats:italic>M. hyorhinis<\/jats:italic> infection on gut microbial composition and metabolome profile, pigs were infected with <jats:italic>M. hyorhinis.<\/jats:italic> Metagenomic sequencing analysis was performed of fecal samples\u00a0and a liquid chromatography\/tandem mass spectrometry (LC\u2013MS\/MS) analysis of gut digesta was made.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pigs infected with <jats:italic>M. hyorhinis<\/jats:italic> had enriched <jats:italic>Sutterella<\/jats:italic> and <jats:italic>Mailhella<\/jats:italic>, and depleted <jats:italic>Dechloromonas<\/jats:italic>, <jats:italic>Succinatimonas<\/jats:italic>, <jats:italic>Campylobacter<\/jats:italic>, <jats:italic>Blastocysti<\/jats:italic>s, <jats:italic>Treponema,<\/jats:italic> and <jats:italic>Megasphaera<\/jats:italic>. The pigs infected with <jats:italic>M. hyorhinis<\/jats:italic> also had greater abundances of <jats:italic>bacterium_0_1xD8_71<\/jats:italic>, <jats:italic>Ruminococcus_sp__CAG_353<\/jats:italic>, <jats:italic>Firmicutes_bacterium_CAG_194<\/jats:italic>, <jats:italic>Firmicutes_bacterium_CAG_534<\/jats:italic>, <jats:italic>bacterium_1xD42_87<\/jats:italic>, and lower abundances of <jats:italic>Chlamydia_suis<\/jats:italic>, <jats:italic>Megasphaera_elsdenii<\/jats:italic>, <jats:italic>Treponema_porcinum<\/jats:italic>, <jats:italic>Bacteroides_sp__CAG_1060<\/jats:italic>, <jats:italic>Faecalibacterium_prausnitzii.<\/jats:italic> Metabolomic analysis revealed that some lipids and lipid\u2010like molecules increased in the small intestine, whereas most lipids and lipid\u2010like molecule metabolites decreased in the large intestine. These altered metabolites induce changes in intestinal sphingolipid metabolism, amino acid metabolism, and thiamine metabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings demonstrate that infection with <jats:italic>M. hyorhinis<\/jats:italic> can alter the gut microbial composition and metabolite structure in pigs, which may further affect amino acid metabolism and lipid metabolism in the intestine. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12690","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T10:50:30Z","timestamp":1683283830000},"page":"6219-6232","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Perturbations of gut microbiome and metabolome of pigs infected with <i>Mycoplasma 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