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The aim of this study was to determine the effects of <jats:italic>in ovo<\/jats:italic> feeding of NAG, 1.5\u2009mg\/egg at 17.5\u2009days of incubation (DOI) via amnion, on hatching performance, early intestinal histomorphometry, jejunal barrier, digestive function, and growth performance of broiler chickens between 1 and 14\u2009days of age.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Amniotic injection of NAG had no significant effect on hatching characteristics compared with the non\u2010injected control group (NC group). Birds in the NAG solution\u2010injected group (NAG group) exhibited lower average daily feed intake and better feed efficiency during a period of 1\u201314\u2009days. In comparison with the NC group, the NAG group had decreased crypt depth (CD) in the ileum and increased villus height (VH) \/ CD in the jejunum at 7\u2009days, and decreased CD in duodenum and significantly increased VH in the jejunum at 14\u2009days. However, the effects of <jats:italic>in ovo<\/jats:italic> supplementation with NAG on the density of goblet cells, and gene expression of <jats:italic>mucin 2<\/jats:italic> and <jats:italic>alkaline phosphatase<\/jats:italic> were not significant. Chicks in the NAG group had a significantly higher mRNA expression level of <jats:italic>trypsin<\/jats:italic> and <jats:italic>maltase<\/jats:italic> in jejunum at 7\u2009days than the NC group but not at 14\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Amniotic injections of NAG (1.5\u2009mg\/egg) at 17.5 DOI could improve early growth performance of broilers during 1\u201314\u2009days after hatching by accelerating the development of the intestine and enhancing jejunal digestive function. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12784","type":"journal-article","created":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T23:05:25Z","timestamp":1686697525000},"page":"6966-6974","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Improvement in growth performance and digestive function from amniotic injections of 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