{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T04:11:21Z","timestamp":1792901481207,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T00:00:00Z","timestamp":1690156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970115"],"award-info":[{"award-number":["31970115"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Enterotoxigenic <jats:italic>Escherichia coli<\/jats:italic> (ETEC) is a pathogen that causes traveler's diarrhea, for which an effective vaccine is lacking. Previous studies showed that <jats:italic>Limosilactobacillus reuteri<\/jats:italic> could inhibit <jats:italic>E. coli<\/jats:italic>, effectively increase the expression of its tight junction protein, and reduce the adhesion of ETEC to the intestinal epithelial Caco\u20102 cell line. In this study, three kinds of yogurt with different starter cultures were first prepared: <jats:italic>Lm<\/jats:italic>. <jats:italic>reuteri<\/jats:italic> yogurt (fermented by <jats:italic>Lm<\/jats:italic>. <jats:italic>reuteri<\/jats:italic> alone), traditional yogurt (fermented by <jats:italic>Streptococcus thermophilus<\/jats:italic> and <jats:italic>Lactobacillus delbrueckii<\/jats:italic> subsp. <jats:italic>bulgaricus<\/jats:italic> at a ratio of 1:1) and mixed yogurt (fermented by <jats:italic>Lm<\/jats:italic>. <jats:italic>reuteri<\/jats:italic>, <jats:italic>S<\/jats:italic>. <jats:italic>thermophilus<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>delbrueckii<\/jats:italic> subsp. <jats:italic>bulgaricus<\/jats:italic> at a ratio of 1:1:1). The physiological properties, oxidative stress, intestinal barrier function, tight junction protein, pathological conditions and intestinal microbiota composition were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The data showed that <jats:italic>Lm<\/jats:italic>. <jats:italic>reuteri<\/jats:italic>\u2010fermented yogurt pregavage could effectively alleviate the intestinal barrier impairment caused by ETEC in mice. It alleviated intestinal villus shortening and inflammatory cell infiltration, decreased plasma diamine oxidase concentration and increased claudin\u20101 and occludin expression in the jejunum of ETEC\u2010infected mice. In addition, <jats:italic>Lm<\/jats:italic>. <jats:italic>reuteri<\/jats:italic>\u2010fermented yogurt significantly reduced the ETEC load in fecal samples, reversed the increase in Pseudomonadota abundance and decreased Bacteroidota abundance caused by ETEC infection. Furthermore, the composition of the intestinal microbiota could maintain a stable state similar to that in healthy mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings indicate that <jats:italic>Lm<\/jats:italic>. <jats:italic>reuteri<\/jats:italic>\u2010fermented yogurt could alleviate intestinal barrier damage, inhibit ETEC growth and maintain the stability of the intestinal microbiota during ETEC infection. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.12836","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T16:28:12Z","timestamp":1688660892000},"page":"7494-7505","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Protective effect of <scp><i>Limosilactobacillus reuteri<\/i><\/scp>\u2010fermented yogurt on mouse intestinal barrier injury induced by enterotoxigenic <scp><i>Escherichia 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