{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T22:40:01Z","timestamp":1774305601457,"version":"3.50.1"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972528"],"award-info":[{"award-number":["31972528"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As indigestible carbohydrates, milk oligosaccharides possess various benefits for newborns, mainly through intestinal microbiota, among which 2'\u2010fucosyllactose (2'\u2010FL) is the most predominant milk oligosaccharide. However, knowledge about the fermentative characteristics of 2'\u2010FL in the gut remains limited, especially in the small intestine. The aim of this study is to explore the differential fermentability of 2'\u2010FL by the small and large intestinal microbiota of piglets using fructo\u2010oligosaccharide (FOS) and lactose\u00a0as controls in an <jats:italic>in vitro<\/jats:italic> batch fermentation experiment. During fermentation, microbial composition was characterized along with gas production and short\u2010chain fatty acid production.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>2'\u2010Fucosyllactose showed differential fermentability in jejunal and colonic fermentation. Compared with the colon, 2'\u2010FL produced less gas in the jejunum than in the FOS and lactose groups (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Meanwhile, 2'\u2010FL exhibited a different influence on the microbial composition and metabolism in the jejunum and colon compared with FOS and lactose. In the jejunum, compared with the FOS and lactose groups, the 2'\u2010FL group showed a higher abundance of <jats:italic>Bacteroides<\/jats:italic>, <jats:italic>Prevotella<\/jats:italic>, and <jats:italic>Blautia,<\/jats:italic> but a lower abundance of <jats:italic>Streptococcus<\/jats:italic> and <jats:italic>Lactobacillus<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), with a higher level of propionate and a lower level of lactate during fermentation (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). In the colon, compared with the FOS and lactose groups, 2'\u2010FL increased the abundance of <jats:italic>Blautia<\/jats:italic>, <jats:italic>Faecalibacterium<\/jats:italic>, and <jats:italic>Lachnospiraceae FCS020,<\/jats:italic> but decreased the abundance of <jats:italic>Prevotella_9<\/jats:italic>, <jats:italic>Succinivibrio<\/jats:italic>, and <jats:italic>Megasphaera<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) with an increase in acetate production (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, the results suggested that the small intestinal microbiota had the potential to ferment milk oligosaccharides. Meanwhile, in comparison with FOS and lactose, 2\u2032\u2010FL selectively stimulated the growth of propionate\u2010producing bacteria in the jejunum and acetate\u2010producing bacteria in the colon. These results demonstrated the differences in fermentation properties of 2'\u2010FL by small and large intestinal microbiota and provided new evidence for the application of 2'\u2010FL in optimizing gut microbiota. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13037","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T23:40:31Z","timestamp":1697067631000},"page":"1553-1563","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of 2'\u2010fucosyllactose on the composition and metabolic activity of intestinal microbiota from piglets after <i>in vitro<\/i> fermentation"],"prefix":"10.1002","volume":"104","author":[{"given":"Yanan","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"},{"name":"National Center for International Research on Animal Gut Nutrition Nanjing Agricultural University  Nanjing China"},{"name":"Key Laboratory of Applied Technology on Green\u2010Eco\u2010Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology &amp; College of Veterinary Medicine Zhejiang A&amp;F University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanxin","family":"Ye","sequence":"additional","affiliation":[{"name":"Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology Nanjing Agricultural University  Nanjing China"},{"name":"National Center for International Research on Animal Gut Nutrition Nanjing Agricultural University  Nanjing 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