{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:18:01Z","timestamp":1789611481707,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1300900"],"award-info":[{"award-number":["2022YFD1300900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The high fibre content of whole plants of <jats:italic>Broussonetia papyrifera<\/jats:italic> limits its efficient utilization. Ferulic acid esterase (FAE), in combination with xylanase, can effectively cleave the lignin\u2013carbohydrate complex, promoting the function of cellulase. However, little is known about the impact of these additives on silage. To effectively utilize natural woody plant resources, FAE\u2010producing <jats:italic>Lactiplantibacillus plantarum<\/jats:italic> RO395, xylanase (XY) and cellulase (CE) were used to investigate the dynamic fermentation characteristics, fibre and nitrogen components and microbial community structure during <jats:italic>B. papyrifera<\/jats:italic> ensiling.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:italic>Broussonetia papyrifera<\/jats:italic> was either not treated (CK) or treated with FAE\u2010producing lactic acid bacteria (LP), CE, XY, LP\u2009+\u2009CE, LP\u2009+\u2009XY or LP\u2009+\u2009CE\u2009+\u2009XY for 3, 7, 15, 30 or 60\u2009days, respectively. In comparison with those in the CK treatment, the <jats:italic>L. plantarum<\/jats:italic> and enzyme treatments (LP\u2009+\u2009CE, LP\u2009+\u2009XY and LP\u2009+\u2009XY\u2009+\u2009CE), especially the LP\u2009+\u2009XY\u2009+\u2009CE treatment, significantly increased the lactic acid concentration and decreased the pH and the contents of acid detergent insoluble protein and NH<jats:sub>3<\/jats:sub>\u2010N (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Enzyme addition improved the degradation efficiency of lignocellulose, and a synergistic effect was observed after enzyme treatment in combination with LP; in addition, the lowest acid detergent fibre, neutral detergent fibre, hemicellulose and cellulose contents were detected after the LP\u2009+\u2009CE\u2009+\u2009XY treatment (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Moreover, CE, XY and LP additions significantly improved the microbial community structure, increased the relative abundance of <jats:italic>Lactiplantibacillus<\/jats:italic> and Firmicutes, and effectively inhibited undesirable bacterial (<jats:italic>Enterobacter<\/jats:italic>) growth during ensiling.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>FAE\u2010producing <jats:italic>L. plantarum<\/jats:italic> and the two tested enzymes exhibited synergistic effects on improving the quality of silage, which indicates that this combination can serve as an efficient method for improved <jats:italic>B. papyrifera<\/jats:italic> silage utilization. \u00a9 2023 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13239","type":"journal-article","created":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T23:05:13Z","timestamp":1703545513000},"page":"3543-3558","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Synergistic effects of ferulic acid esterase\u2010producing lactic acid bacteria, cellulase and xylanase on the fermentation characteristics, fibre and nitrogen components and microbial community structure of <i>Broussonetia papyrifera<\/i> during ensiling"],"prefix":"10.1002","volume":"104","author":[{"given":"Qiang","family":"Yu","sequence":"first","affiliation":[{"name":"College of Animal Science Guizhou University  Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyi","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science Guizhou University  Guizhou 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