{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:39:06Z","timestamp":1791279546460,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Alcoholic liver disease (ALD) is responsible for 3.3 million deaths per annum. Efficacious therapeutic modalities or drug treatments for ALD have not yet been found, so it is urgent to seek new agents for preventing ALD and its related disease. Many experiments have indicated that modulating the gut microbiota and regulating the toll\u2010like receptor 4 (TLR<jats:sub>4<\/jats:sub>)\/nuclear transcription factor\u2010\u03baB (NF\u2010\u03baB) inflammatory pathway can provide a new target for prevention and treatment of ALD. Marine microalgae have their natural metabolic pathways to synthesize various of bioactive compounds as promising candidates for hepatoprotection. In this study, we investigated ethanol extracts from <jats:italic>Isochrysis zhanjiangensis<\/jats:italic> (EEIZ) to evaluate their ability to alleviate acute alcoholic liver injury, regulate TLR<jats:sub>4<\/jats:sub>\/NF\u2010\u03baB inflammatory pathway and modulate intestinal bacteria dysbiosis in mice for ALD treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In the acute ALD mouse model, EEIZ reduced levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, triacylglyceride, total cholesterol and low\u2010density lipoprotein, while increasing the level of high\u2010density lipoprotein. Besides, TLR<jats:sub>4<\/jats:sub>, myeloid differentiation factor 88, NF\u2010\u03baB and tumor necrosis factor\u2010\u03b1 expression levels in liver tissue were effectively downregulated by EEIZ. Furthermore, treatment with EEIZ enhanced intestinal homeostasis and significantly alleviated the damage caused by alcohol.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>EEIZ showed effective hepatoprotective activity against alcohol\u2010induced acute liver injury in mice as it could alleviate hepatocyte damage, suppress the TLR<jats:sub>4<\/jats:sub>\/NF\u2010\u03baB inflammatory pathway and regulate the intestinal flora structure. EEIZ could be a good candidate for preventing acute alcoholic liver injury. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13321","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T05:26:57Z","timestamp":1707283617000},"page":"4354-4362","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Ethanol extracts of <i>Isochrysis zhanjiangensis<\/i> alleviate acute alcoholic liver injury and modulate intestinal bacteria dysbiosis in mice"],"prefix":"10.1002","volume":"104","author":[{"given":"Yangmin","family":"Wen","sequence":"first","affiliation":[{"name":"Department of basic medical science Quanzhou Medical College  Quanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5243-749X","authenticated-orcid":false,"given":"Youcai","family":"Zhou","sequence":"additional","affiliation":[{"name":"School 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