{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:29:47Z","timestamp":1792024187129,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1000500"],"award-info":[{"award-number":["2023YFD1000500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>It is reported that anti\u2010enterovirus 71 (EV71) drugs have some side effects on human health. Notably, fungi plays a crucial role in promoting human health and anti\u2010virus. <jats:italic>Grifola frondosa<\/jats:italic> is a type of large medicinal and edible fungi, rich in active substances. The present study aimed to investigate the anti\u2010EV71 effect of <jats:italic>G. frondosa<\/jats:italic> and the potential active substances.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In the present study, the water extract of <jats:italic>G. frondosa<\/jats:italic> was subjected to ethanol precipitation to obtain the water\u2010extracted supernatant of <jats:italic>G. frondosa<\/jats:italic> (GFWS) and water\u2010extracted precipitation of <jats:italic>G. frondosa<\/jats:italic>. Their inhibitory effects on EV71 virus were studied based on a cell model. The results showed that GFWS had stronger security and anti\u2010EV71 effects. In addition, the chemical constituents of GFWS were identified by ultra\u2010high performance liquid chromatography\u2010tandem mass spectrometry, which were selected for further separation and purification. Three compounds, <jats:italic>N<\/jats:italic>\u2010butylaniline, succinic acid and <jats:sc>l<\/jats:sc>\u2010tryptophan, were isolated from GFWS by NMR spectroscopy. It is noteworthy that <jats:italic>N<\/jats:italic>\u2010butylaniline and <jats:sc>l<\/jats:sc>\u2010tryptophan were isolated and identified from the <jats:italic>G. frondosa<\/jats:italic> fruiting bodies for the first time. Our study found that <jats:sc>l<\/jats:sc>\u2010tryptophan has anti\u2010EV71 virus activity, which reduced EV71\u2010induced apoptosis and significantly inhibited the replication process after virus adsorption. Furthermore, it could also bind to capsid protein VP1 to prevent the virus from attaching to the cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:sc>l<\/jats:sc>\u2010tryptophan was an inhibitor of the EV71 virus, which could be used in infant nutrition and possibly provide a new drug to treat hand, foot and mouth disease. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13332","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T07:58:47Z","timestamp":1707292727000},"page":"4453-4464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Isolation and identification of active components from <i>Grifola frondosa<\/i> and its <scp>anti\u2010EV71<\/scp> virus 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