{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:57:49Z","timestamp":1792173469229,"version":"4.4.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The present study was conducted to investigate the effects of dietary novel alkaline protease from <jats:italic>Bacillus licheniformis<\/jats:italic> on the growth performance, meat quality, antioxidant status and intestinal morphology of broilers. In total, 4000 broilers were randomly assigned into five groups and treated with normal control, normal control + 100\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> protease, normal control + 200\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> protease, normal control + 300\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> protease and normal control + 400\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> protease.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Supplementing protease impacted final body weight (linear, <jats:italic>P<\/jats:italic>\u2009=\u20090.003; quadratic, <jats:italic>P<\/jats:italic>\u2009=\u20090.006) and decreased feed conversion rate (linear, <jats:italic>P<\/jats:italic>\u2009=\u20090.036) in broilers. Moreover, dietary protease significantly increased breast muscle rate (linear, <jats:italic>P<\/jats:italic>\u2009=\u20090.005; quadratic, <jats:italic>P<\/jats:italic>\u2009=\u20090.021) and decreased drip loss (linear, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001; quadratic, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). In addition, dietary protease notably increased protein digestibility (linear, <jats:italic>P<\/jats:italic>\u2009=\u20090.001; quadratic, <jats:italic>P<\/jats:italic>\u2009=\u20090.006) and trypsin activity (linear, <jats:italic>P<\/jats:italic>\u2009=\u20090.002; quadratic, <jats:italic>P<\/jats:italic>\u2009=\u20090.009) in jejunum. Light microscopy revealed that the jejunum villi in the 300\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> and 400\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> groups exhibited greater height and a denser arrangement compared to those in the control group. The addition of protease decreased malondialdehyde content (linear, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001; quadratic, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and increased total antioxidant capacity (linear, <jats:italic>P<\/jats:italic>\u2009=\u20090.001; quadratic, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) in pectoral muscles.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The results of the present study suggest that dietary novel alkaline protease from <jats:italic>B.\u2009licheniformis<\/jats:italic> improved growth performance by affecting trypsin activity, protein digestibility, antioxidant capacity and intestinal health. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13337","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T08:09:30Z","timestamp":1706515770000},"page":"5176-5185","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effect of a novel alkaline protease from <scp><i>Bacillus licheniformis<\/i><\/scp> on growth performance, carcass characteristics, meat quality, antioxidant capacity, and intestinal morphology of white feather broilers"],"prefix":"10.1002","volume":"104","author":[{"given":"Wuzhou","family":"Yi","sequence":"first","affiliation":[{"name":"College of Animal Sciences Zhejiang University  Hangzhou China"},{"name":"The Key Laboratory of Molecular Animal Nutrition Ministry of Education  Hangzhou China"},{"name":"Zhejiang Provincial Laboratory of Feed and Animal Nutrition  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjie","family":"Liu","sequence":"additional","affiliation":[{"name":"Jinan Bestzyme Bio\u2010Engineering Co., Ltd  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijun","family":"Fu","sequence":"additional","affiliation":[{"name":"Shandong Binzhou Animal Science&amp; 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