{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T23:07:24Z","timestamp":1778800044554,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T00:00:00Z","timestamp":1709510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171475"],"award-info":[{"award-number":["32171475"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20210465"],"award-info":[{"award-number":["BK20210465"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Acidic lipases with high catalytic activities under acidic conditions have important application values in the food, feed and pharmaceutical industries. However, the availability of acidic lipases is still the main obstacle to their industrial applications. Although a novel acidic lipase <jats:italic>Rasamsonia emersonii<\/jats:italic> (LIPR) was heterologously expressed in <jats:italic>Escherichia coli<\/jats:italic>, the expression level was unsatisfactory.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>To achieve the high\u2010efficiency expression and secretion of LIPR in <jats:italic>Pichia pastoris<\/jats:italic> GS115, the combinatorial optimization strategy was adopted including gene codon preference, signal peptide, molecular chaperone co\u2010expression and disruption of vacuolar sorting receptor <jats:italic>VPS10<\/jats:italic>. The activity of the combinatorial optimization engineered strain in a shake flask reached 1480\u2009U\u2009mL<jats:sup>\u22121<\/jats:sup>, which was 8.13 times greater than the <jats:italic>P. pastoris<\/jats:italic> GS115 parental strain. After high\u2010density fermentation in a 5\u2010L bioreactor, the highest enzyme activity reached as high as 11\u2009820\u2009U\u2009mL<jats:sup>\u22121<\/jats:sup>. LIPR showed the highest activity at 40\u2009\u00b0C and pH\u20094.0 in the presence of Ca<jats:sup>2+<\/jats:sup> ion. LIPR exhibited strong tolerance to methanol, indicating its potential application in biodiesel biosynthesis. Moreover, the gastrointestinal digestion simulation results demonstrated that LIPR was tolerant to pepsin and trypsin, but its activity was inhibited by sodium taurodeoxycholate.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study provided an effective approach for the high expression of acidic lipase LIPR. LIPR was more appropriate for lipid digestion in the stomach than in intestine according to the gastrointestinal digestion simulation results. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13390","type":"journal-article","created":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T08:48:45Z","timestamp":1708073325000},"page":"5603-5613","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Highly efficient expression of <i>Rasamsonia emersonii<\/i> lipase in <i>Pichia pastoris<\/i>: characterization and gastrointestinal simulated digestion <i>in vitro<\/i>"],"prefix":"10.1002","volume":"104","author":[{"given":"Buqing","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education School of Biotechnology, Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasen","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education School of Biotechnology, Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoman","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education School of Biotechnology, Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Dong","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biochemical Engineering Institute of Process Engineering, Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morihisa","family":"Fujita","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education School of Biotechnology, Jiangnan University  Wuxi China"},{"name":"Institute for Glyco\u2010Core Research, Gifu University  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5618-1862","authenticated-orcid":false,"given":"Zijie","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education School of Biotechnology, Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2017.07.014"},{"key":"e_1_2_8_3_1","first-page":"36","article-title":"Expression of aspergillus fumigates lipase B in Pichia pastoris and biochemical characterization","volume":"2","author":"Tang W","year":"2019","journal-title":"J Food Sci Technol"},{"key":"e_1_2_8_4_1","first-page":"70","article-title":"Recombination expression and enzymatic characteristics of mono\u2010 and diacylglycerol lipase PrLip","volume":"4","author":"Liu X\u2010H","year":"2020","journal-title":"J Food Sci Technol"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.05.199"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.09.020"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-010-0700-2"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2014.03.004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12088-011-0087-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798316008482"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00304"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-022-01904-3"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab535"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-019-1597-8"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-022-02243-6"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2017.11.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.202300259"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-018-1531-5"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26440"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-16104-4"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.0c00641"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms15010203"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2022.126769"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.2c00328"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12020"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5844"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112295200"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2013.06.017"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2022.110098"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202107148"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-010-0159-z"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-015-0225-5"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2016.05.003"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2014.10.019"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0FO02976A"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1142536"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.11.050"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.127225"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5FO00930H"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T400003-MCP200"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/77.11.1665"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2016.09.040"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-013-2354-x"},{"key":"e_1_2_8_44_1","first-page":"100","article-title":"Thermostable acidic lipase of Bacillus glycinifermentans\u2010MK840989 isolated from contaminated environment; its optimization, purification and exploring potential applications","volume":"5","author":"Shamim S","year":"2020","journal-title":"Moj Ecology Environ Sci"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-013-1355-6"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-020-02976-2"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11756-021-01005-3"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.13390","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:41:25Z","timestamp":1760881285000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.13390"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,4]]},"references-count":46,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1002\/jsfa.13390"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.13390","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,4]]},"assertion":[{"value":"2023-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}