{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T17:39:39Z","timestamp":1788629979470,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"MOST","doi-asserted-by":"publisher","award":["2018YFE0108400."],"award-info":[{"award-number":["2018YFE0108400."]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Flax lignan has attracted much attention because of its potential bioactivities. However, the bioavailability of secoisolariciresinol diglucoside (SDG), the main lignan in flaxseed, depends on the bioconversion by the colon bacteria. Lactic acid bacteria (LAB) with <jats:italic>\u03b2<\/jats:italic>\u2010glucosidase activity has found wide application in preparing bioactive aglycone.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>LAB strains with good <jats:italic>\u03b2<\/jats:italic>\u2010glucosidase activity were isolated from fermented tofu. Their bioconversion of flax lignan extract was investigated by resting cell catalysis and microbial fermentation, and the metabolism of SDG by <jats:italic>Lactiplantibacillus plantarum<\/jats:italic> C5 following fermentation was characterized by widely targeted metabolomics. Five <jats:italic>L. plantarum<\/jats:italic> strains producing <jats:italic>\u03b2<\/jats:italic>\u2010glucosidase with broad substrate specificity were isolated and identified, and they all can transform SDG into secoisolariciresinol (SECO). <jats:italic>L. plantarum<\/jats:italic> C5 resting cell reached a maximum SDG conversion of 49.19\u2009\u00b1\u20093.75%, and SECO generation of 21.49\u2009\u00b1\u20091.32% (0.215\u2009\u00b1\u20090.013\u2009m<jats:sc>m<\/jats:sc>) at an SDG substrate concentration of 1\u2009mM and 0.477\u2009\u00b1\u20090.003\u2009m<jats:sc>m<\/jats:sc> SECO was produced at 4\u2009m<jats:sc>m<\/jats:sc> within 24\u2009h. Although sixteen flax lignan metabolites were identified following the fermentation of SDG extract by <jats:italic>L. plantarum<\/jats:italic> C5, among them, four were produced following the fermentation: SECO, demethyl\u2010SECO, demethyl\u2010dehydroxy\u2010SECO and isolariciresinol. Moreover, seven lignans increased significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Fermentation significantly increased the profile and level of flax lignan metabolites, and the resting cell catalysis benefits from higher bioconversion efficiency and more straightforward product separation. Resting cell catalysis and microbial fermentation of flax lignan extract by the isolated <jats:italic>\u03b2<\/jats:italic>\u2010glucosidase production <jats:italic>L. plantarum<\/jats:italic> could be potentially applied in preparing flax lignan ingredients and fermented flaxseed. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13412","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T05:43:31Z","timestamp":1708926211000},"page":"5869-5881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A comparative study on flaxseed lignan biotransformation through resting cell catalysis and microbial fermentation by <i>\u03b2<\/i>\u2010glucosidase production <scp><i>Lactiplantibacillus plantarum<\/i><\/scp>"],"prefix":"10.1002","volume":"104","author":[{"given":"Chengcheng","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Food Science and Engineering Jinan University  Guangzhou China"},{"name":"Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition and Safety, Department of Food Science and Engineering Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Food Science and Engineering Jinan University  Guangzhou China"},{"name":"Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition and Safety, Department of Food Science and Engineering Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zizhe","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Food Science and Engineering Jinan University  Guangzhou China"},{"name":"Guangdong Joint International Research Centre of Oilseed Biorefinery, Nutrition and Safety, Department of Food Science and Engineering Jinan 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