{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T14:27:15Z","timestamp":1786199235220,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972598"],"award-info":[{"award-number":["31972598"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Early weaning is prone to damage intestinal barrier function, resulting in diarrhea, whereas rutin, as a natural flavonoid with multiple biological functions, shows potential in piglets. Therefore, the effects of dietary rutin on growth, antidiarrheal, barrier function, antioxidant status and cecal microbiota of weaned piglets were investigated with the control group (CON) (basal diet) and Rutin (basal diet+500\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> rutin) groups fed for 14\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The results showed that dietary 500\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> rutin significantly decreased diarrhea index, serum diamine oxidase activity and total aerobic bacterial population in mesenteric lymph nodes, whereas it significantly increased the gain\u2010to\u2010feed ratio (G:F) and serum growth hormone content, jejunal villus height and villus height to crypt depth ratio, and also enhanced jejunal claudin\u20101 and zonula occludens\u20101 mRNA and protein expression. Meanwhile, dietary rutin significantly decreased inflammation\u2010associated mRNA expression, malondialdehyde (MDA) content, swollen mitochondrial number and mitochondrial area in the jejunum, whereas it increased the total superoxide dismutase (T\u2010SOD) and glutathione peroxidase activities and activated the Nrf2 signaling pathway. Moreover, dietary rutin significantly increased <jats:italic>Firmicutes<\/jats:italic> abundance and decreased <jats:italic>Campylobacterota<\/jats:italic> abundance, which were closely associated with the decreased diarrhea index and MDA content or increased Claudin\u20101 expression and T\u2010SOD activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Dietary 500\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> rutin increased G:F by improving intestinal morphology, and alleviated diarrhea by enhancing intestinal barrier, which might be associated with the enhanced antioxidant capacity via activating the Nrf2\/Keap1 signaling pathway and the improved cecal microbial composition in weaned piglets. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13456","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T09:49:23Z","timestamp":1710150563000},"page":"6262-6275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Dietary rutin improves the antidiarrheal capacity of weaned piglets by improving intestinal barrier function, antioxidant capacity and cecal microbiota composition"],"prefix":"10.1002","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8597-104X","authenticated-orcid":false,"given":"Longfei","family":"Ma","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binbin","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Nanjing Agricultural University  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