{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T11:14:20Z","timestamp":1778152460947,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T00:00:00Z","timestamp":1711756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD2101201"],"award-info":[{"award-number":["2022YFD2101201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Rice\u2010based distillers' spent cake (RDSC), a by\u2010product of the Chinese liquor (Baijiu) industry, is a potential source of angiotensin\u2010converting enzyme (ACE) inhibitory peptide. Since ACE plays a crucial role in controlling hypertension, inhibition of ACE has been widely emphasized. The ACE inhibitory active peptide derived from by\u2010products of food has been recognized as a safer and cheaper inhibitor.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Aimed to discover ACE\u2010inhibiting active peptides in RDSC. Hydrolysis of RDSC by alcalase for 4\u2009h followed by ultrafiltration yielded low\u2010molecular\u2010weight (&lt;\u20093\u2009kDa) fractions. Subsequently, a comprehensive method using a combination of liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry (LC\u2010Q\u2010TOF\u2010MS) and LC\u2010Q\u2010Exactive\u2010MS to identify the novel short peptides (3\u20135 amino acids residues; <jats:italic>n<\/jats:italic>\u2009=\u20097) and medium\u2010sized peptides (more than 6 amino acids residues; <jats:italic>n<\/jats:italic>\u2009=\u20096). <jats:italic>In vitro<\/jats:italic> activity assay showed that the peptides KPFFPGL, GFPRPLL, GPPGVF, and VGK exhibited the highest activity with inhibitory concentration of 50% (IC<jats:sub>50<\/jats:sub>) of 11.63, 12.34, 19.55, and 33.54\u2009\u03bcmol L<jats:sup>\u22121<\/jats:sup>. Molecular docking reveal that the active and inactive sites (Glu123, Asp121, Arg522, and Lys118) play important roles in enhancing the ACE inhibitory activity of peptides.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Here we report a comprehensive method that effectively extracted and identified the bioactive peptides from RDSC. Four highly active novel peptides may be the most promising candidates for functional foods against hypertension, provide significant information for enhancing value of rice\u2010based distilled by\u2010products. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13474","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T13:09:36Z","timestamp":1710940176000},"page":"6506-6517","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Preparation, identification, and molecular docking of novel angiotensin\u2010converting enzyme inhibitory peptides derived from rice\u2010based distillers' spent cakes"],"prefix":"10.1002","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3554-0966","authenticated-orcid":false,"given":"Hanxing","family":"Su","sequence":"first","affiliation":[{"name":"Laboratory of Brewing Microbiology and Applied Enzymology, Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlai","family":"Fan","sequence":"additional","affiliation":[{"name":"Laboratory of Brewing Microbiology and Applied Enzymology, Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7919-4762","authenticated-orcid":false,"given":"Yan","family":"Xu","sequence":"additional","affiliation":[{"name":"Laboratory of Brewing Microbiology and Applied Enzymology, Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology Jiangnan University  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaopei","family":"Tang","sequence":"additional","affiliation":[{"name":"Baijiu Fermentation Technology Research Center of Jiangnan University\u2010Kweichow Zhen Distillery Co. Ltd  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dehong","family":"Yue","sequence":"additional","affiliation":[{"name":"Baijiu Fermentation Technology Research Center of Jiangnan University\u2010Kweichow Zhen Distillery Co. Ltd  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuyue","family":"Liao","sequence":"additional","affiliation":[{"name":"Baijiu Fermentation Technology Research Center of Jiangnan University\u2010Kweichow Zhen Distillery Co. Ltd  Wuxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7060469"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.135372"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12601"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2017.1374923"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.7379"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2019.07.041"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2007.01.013"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2019.05.091"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2021.129521"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201000329"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1271\/nogeikagaku1924.66.1081"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijfs.14495"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.8523"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2021.112221"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods8090390"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.58.1767"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c07270"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14204400"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2021.104892"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.11445"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c02487"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128443"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9091185"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.68.551"},{"key":"e_1_2_9_26_1","volume-title":"Cereals and Pulses \u2014 Determination of the Nitrogen Content and Calculation of the Crude Protein Content","author":"ISO, 20483:2013","year":"2013"},{"key":"e_1_2_9_27_1","volume-title":"Cereals, Pulses and by\u2010Products \u2014 Determination of Ash Yield by Incineration","author":"ISO, 2171:2023","year":"2023"},{"key":"e_1_2_9_28_1","volume-title":"Cereals, Cereals\u2010Based Products and Animal Feeding Stuffs \u2014 Determination of Crude Fat and Total Fat Content by the Randall Extraction Method","author":"ISO, 11085:2015","year":"2015"},{"key":"e_1_2_9_29_1","volume-title":"Animal Feeding Stuffs \u2014 Enzymatic Determination of Total Starch Content","author":"ISO, 15914:2004","year":"2004"},{"key":"e_1_2_9_30_1","volume-title":"Agricultural Food Products \u2014 Determination of Crude Fibre Content; (Modified Scharrer Method)","author":"ISO, 6541:1981","year":"1981"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2016.04.031"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1141"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj008"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2018.12.015"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12512"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6CP01555G"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11746-011-1941-6"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1590\/0104-6632.20190364s20180599"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf072238d"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00217-020-03552-6"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.7b04815"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.02.018"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26061515"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2022.100231"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b01922"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.04.166"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2023.03.006"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2015.1007167"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12081573"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.13474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:32:07Z","timestamp":1760884327000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.13474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,30]]},"references-count":48,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,8,30]]}},"alternative-id":["10.1002\/jsfa.13474"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.13474","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,30]]},"assertion":[{"value":"2023-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}