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Some <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> studies have demonstrated that \u2018non\u2010digestible\u2019 oligosaccharides are digested to different degrees depending on their structural composition. In the present study, we compared different methods aiming to assess the digestibility of oligosaccharides synthesized by <jats:italic>\u03b2<\/jats:italic>\u2010galactosidase (<jats:italic>\u03b2<\/jats:italic>\u2010gal) of <jats:italic>Lactobacillus delbruecki<\/jats:italic> subsp. <jats:italic>bulgaricus<\/jats:italic> CRL450 (CRL450\u2010<jats:italic>\u03b2<\/jats:italic>\u2010gal) from lactose, lactulose and lactitol.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In the simulated gastrointestinal fluid method, no changes were observed. However, the oligosaccharides synthesized by CRL450\u2010<jats:italic>\u03b2<\/jats:italic>\u2010gal were partially hydrolyzed <jats:italic>in vitro<\/jats:italic>, depending on their structure and composition, with rat small intestinal extract (RSIE) and small intestinal brush\u2010border membrane vesicles (BBMV) from pig. Digestion of some oligosaccharides increased when mixtures were fed to C57BL\/6 mice used as <jats:italic>in vivo<\/jats:italic> model; however, lactulose\u2010oligosaccharides were the most resistant to the physiological conditions of mice. In general <jats:italic>\u03b2<\/jats:italic> (1\u21926) linked products showed higher resistance compared to <jats:italic>\u03b2<\/jats:italic> (1\u21923) oligosaccharides.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p><jats:italic>In vitro<\/jats:italic> digestion methods, without disaccharidases, may underestimate the importance of carbohydrates hydrolysis in the small intestine. Although BVMM and RSIE digestion assays are appropriate <jats:italic>in vitro<\/jats:italic> methods for these studies, <jats:italic>in vivo<\/jats:italic> studies remain the most reliable for understanding what actually happens in the digestion of oligosaccharides. \u00a9 2024 The Authors. <jats:italic>Journal of The Science of Food and Agriculture<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13504","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T11:03:57Z","timestamp":1712055837000},"page":"6769-6777","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i> and <i>in vivo<\/i> digestibility of prebiotic galactooligosacharides synthesized by <i>\u03b2<\/i>\u2010galactosidase from <i>Lactobacillus delbruecki<\/i> subsp. <i>bulgaricus<\/i><scp>CRL450<\/scp>"],"prefix":"10.1002","volume":"104","author":[{"given":"Agustina","family":"Fara","sequence":"first","affiliation":[{"name":"Laboratorio de Ecofisiolog\u00eda Tecnol\u00f3gica CERELA\u2010CONICET  San Miguel de Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oswaldo","family":"Hern\u00e1ndez Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica y Funcionalidad de Carbohidratos y Derivados Instituto de Investigaci\u00f3n en Ciencias de la Alimentaci\u00f3n (CIAL) (CSIC\u2010UAM)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Palacios","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecofisiolog\u00eda Tecnol\u00f3gica CERELA\u2010CONICET  San Miguel de Tucum\u00e1n Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2117-6999","authenticated-orcid":false,"given":"Antonia","family":"Montilla","sequence":"additional","affiliation":[{"name":"Grupo de Qu\u00edmica y Funcionalidad de Carbohidratos y Derivados Instituto de Investigaci\u00f3n en Ciencias de la Alimentaci\u00f3n (CIAL) (CSIC\u2010UAM)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5349-1067","authenticated-orcid":false,"given":"Gabriela","family":"Z\u00e1rate","sequence":"additional","affiliation":[{"name":"Laboratorio de Ecofisiolog\u00eda Tecnol\u00f3gica CERELA\u2010CONICET  San Miguel de Tucum\u00e1n Argentina"},{"name":"Universidad de San Pablo Tucum\u00e1n Av. 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