{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T03:55:26Z","timestamp":1785038126450,"version":"3.55.0"},"reference-count":41,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2024,12]]},"abstract":"Abstract<\/jats:title>BACKGROUND<\/jats:title>Deer\u2010derived materials (antler, venison, fetus, penis, bone, tail, and others) are some of the most valuable traditional animal\u2010based medicinal and food materials in China. In production, processing, and trade, the quality of deer products varies. The market is confusing, and counterfeit and shoddy products are common. There is an urgent need to establish an accurate identification method.<\/jats:p><\/jats:sec>RESULTS<\/jats:title>Two pairs of primers suitable for identifying deer\u2010derived medicinal materials were obtained by screening the cytochrome oxidase I (COI) sequences of 18 species from nine genera of the deer family. The two primers were used to identify the species and adulteration of 22 batches of commercially available deer\u2010derived products with a mini\u2010barcode combining high\u2010resolution melting (HRM) technology and methodical investigation. Deer\u2010derived materials (sika and red deer) were correctly identified by species using varying DNA amounts (1 to 500 ng). The two pairs of primers COI\u20101FR and COI\u20102FR yielded melting temperatures (Tm) of 80.55 to 81.00\u2009\u00b0C and 82.00 to 82.50\u2009\u00b0C for sika deer, and 81.00 to 82.00\u2009\u00b0C and 81.40 to 82.00\u2009\u00b0C for red deer. Twenty\u2010two batches of commercially available samples were analyzed by HRM analysis and conventional amplification sequencing, and it was found that the species samples had an error rate of species labeling of 31.8%. Four batches of samples were identified as mixed (adulterated) in the HRM analysis.<\/jats:p><\/jats:sec>CONCLUSION<\/jats:title>The combination of DNA mini\u2010barcode with HRM analysis facilitated the accurate identification of species of deer\u2010derived materials, especially the identification of samples in an adulterated mixed state. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13761","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T08:44:31Z","timestamp":1721897071000},"page":"9390-9398","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["High\u2010resolution melting analysis to authenticate deer\u2010derived materials in processed products in China using a cytochrome oxidase I 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anzhen","family":"Xie","sequence":"additional","affiliation":[{"name":"Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & International Joint Research Center for Quality of Traditional Chinese Medicine, Hainan Branch of the Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixiao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & International Joint Research Center for Quality of Traditional Chinese Medicine, Hainan Branch of the Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Haikou 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