{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:51:59Z","timestamp":1782363119557,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T00:00:00Z","timestamp":1726704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Cyclocarya paliurus<\/jats:italic>, as a new food resource, is utilized extensively in human and animal diets due to its bioactive compounds, health benefits, and its highly prized sweet flavor. This study aimed to investigate the sweet\u2010taste ingredient of <jats:italic>C. paliurus<\/jats:italic> leaves.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Five new dammarane triterpenoid glycosides were isolated and identified as qingqianliutianosides A\u2013E (<jats:bold>1<\/jats:bold>\u2013<jats:bold>5<\/jats:bold>) by comprehensive spectroscopic data analysis and a single crystal X\u2010ray diffraction experiment. Qingqianliutianoside A (<jats:bold>1<\/jats:bold>) and qingqianliutianoside C (<jats:bold>3<\/jats:bold>), present in relatively high quantities in the plant, were shown to exhibit sweetness by sensory evaluation and electronic tongue analysis. Further monitoring was conducted on the content changes in <jats:bold>3<\/jats:bold> in leaves at different growth stages, indicating that <jats:bold>3<\/jats:bold> reached its peak content in April and then showed a decreasing trend. Molecular docking studies revealed that T1R2\/T1R3 receptors Ser212, Ser105, Thr239, Asn380, Thr305, and Val381 may play critical roles, demonstrating that hydrogen bonding and hydrophobic interactions were the dominant interaction forces between all of the identified compounds and the active sites in the Venus flytrap module of the T1R2\/T1R3 receptors.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Qingqianliutianosides A\u2013E are promising natural source sugar substitutes for use in functional foods and beverages. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13912","type":"journal-article","created":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T22:35:32Z","timestamp":1726785332000},"page":"1216-1227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Qingqianliutianosides A\u2013E: five new sweet dammarane triterpenoid glycosides derived from the leaves of <scp><i>Cyclocarya paliurus<\/i><\/scp> \u2013 identification, characterization and interactions with <scp>T1R2<\/scp>\/<scp>T1R3<\/scp> sweet taste receptors"],"prefix":"10.1002","volume":"105","author":[{"given":"Tingsi","family":"Guo","sequence":"first","affiliation":[{"name":"TCM and Ethnomedicine Innovation &amp; Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy Hunan University of Chinese Medicine  Changsha People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Yang","sequence":"additional","affiliation":[{"name":"TCM and Ethnomedicine Innovation &amp; Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy Hunan University of Chinese Medicine  Changsha People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Liang","sequence":"additional","affiliation":[{"name":"TCM and Ethnomedicine Innovation &amp; Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy Hunan University of Chinese Medicine  Changsha People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Huang","sequence":"additional","affiliation":[{"name":"TCM and Ethnomedicine Innovation &amp; 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