{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:15:57Z","timestamp":1784862957825,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,21]],"date-time":"2024-09-21T00:00:00Z","timestamp":1726876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32300328"],"award-info":[{"award-number":["32300328"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004480","name":"Natural Science Foundation of Shanxi Province","doi-asserted-by":"publisher","award":["202203021221024"],"award-info":[{"award-number":["202203021221024"]}],"id":[{"id":"10.13039\/501100004480","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,1,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Nonalcoholic fatty liver disease (NAFLD), a chronic metabolic disorder marked by excessive lipid deposition, represents a considerable health burden with no established efficacious treatment strategy. Quinoa (<jats:italic>Chenopodium quinoa<\/jats:italic> Willd.), valued for its health benefits, is replete with flavonoid bioactives. The aims of this work are to isolate and purify flavonoids from quinoa whole grain that can intervene in NAFLD and to elucidate some of the underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:italic>Chenopodium quinoa<\/jats:italic> Willd. flavonoids (CQWF) were obtained successfully through an optimized ultrasonic extraction methodology, followed by isolation and purification utilizing macroporous resin D101. The study then explored the therapeutic potential of CQWF and its eluted fractions in models emulating NAFLD conditions: an <jats:italic>in vitro<\/jats:italic> fatty liver cell model induced by oleic acid (OA) and palmitic acid (PA) in the HepG2 and BEL\u20107402 cell lines, and an <jats:italic>in vivo<\/jats:italic> high\u2010fat diet (HFD)\u2010induced NAFLD model in C57BL\/6N mice. The findings revealed a comprehensive mitigating effect of CQWF30 on NAFLD, manifesting in reduced intracellular lipid accumulation in steatotic hepatocytes and a concerted downregulation of key lipid metabolism genes, <jats:italic>CD36<\/jats:italic> and <jats:italic>FASN<\/jats:italic>. Administration of CQWF30 reduced triglyceride (TG) levels in both the cellular model and the livers of HFD\u2010fed mice. It also reduced serum concentrations of TG, total cholesterol (T\u2010CHO), low\u2010density lipoprotein cholesterol (LDL\u2010C), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), while increasing high\u2010density lipoprotein cholesterol (HDL\u2010C) in the mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results highlighted the promising therapeutic capacity of CQWF, particularly CQWF30. This research advances the exploration and utilization of flavonoids derived from quinoa whole grain, providing innovative dietary intervention strategies for NAFLD. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13923","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:56:55Z","timestamp":1733878615000},"page":"1330-1342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Isolation and purification of flavonoids from quinoa whole grain and its inhibitory effect on lipid accumulation in nonalcoholic fatty liver disease by inhibiting the expression of <scp>CD36<\/scp> and <scp>FASN<\/scp>"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4680-8200","authenticated-orcid":false,"given":"Xiaoqin","family":"La","sequence":"first","affiliation":[{"name":"Institutes of Biomedical Sciences Shanxi University  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