{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T17:42:29Z","timestamp":1773942149259,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T00:00:00Z","timestamp":1730073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The phytopathogenic genus <jats:italic>Fusarium<\/jats:italic> can cause damage such as root and stem rot in economically important crops, with significant implications. To seek a sustainable method for controlling this phytopathogen in seeds, the antifungal activity of essential oils (EOs) from thyme (<jats:italic>Thymus vulgaris<\/jats:italic>) and oregano (<jats:italic>Origanum vulgare<\/jats:italic>) was evaluated against isolates of <jats:italic>F. graminearum<\/jats:italic>, <jats:italic>F. equiseti<\/jats:italic>, <jats:italic>F. culmorum<\/jats:italic> and <jats:italic>F. oxysporum<\/jats:italic> originating from quinoa (<jats:italic>Chenopodium quinoa<\/jats:italic>) crops in the Boyac\u00e1 (Colombia).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Initially, the effectiveness of commercial fungicides against the mentioned phytopathogenic fungi was evaluated. Upon verifying that these isolates exhibited high resistance to these compounds, the EOs were assessed as a potential control alternative. A disk diffusion assay demonstrated complete <jats:italic>in vitro<\/jats:italic> inhibition of the growth of the evaluated phytopathogens when undiluted EOs were used. Subsequently, the minimum inhibitory concentration (MIC) of these oils was determined using the agar well diffusion technique, revealing a MIC of 10 and 1\u2009\u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup> for thyme and oregano oil, respectively. Following this, the antifungal activity of the EOs applied to quinoa seeds was evaluated, and germination indices were measured as an indirect indicator of their toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Despite both EOs successfully inhibiting microbial growth in the seeds, it was also found that thyme EO at 100\u2009\u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup> and oregano EO at 10\u2009\u03bcL\u2009mL<jats:sup>\u22121<\/jats:sup> inhibited seed emergence and germination. However, lower concentrations exhibited a reduction in fungal population without affecting these germination indices. Therefore, it is suggested that the use of these compounds has potential in the treatment and disinfection of quinoa seeds. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.13993","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T06:33:53Z","timestamp":1730097233000},"page":"2236-2245","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Essential oils of thyme (<scp><i>Thymus vulgaris<\/i><\/scp>) and oregano (<scp><i>Origanum vulgare<\/i><\/scp>) as an alternative for the control of pesticide\u2010resistant <scp><i>Fusarium<\/i> spp<\/scp>. in quinoa seeds"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1037-4984","authenticated-orcid":false,"given":"Ingrid Rocio","family":"Fonseca\u2010Guerra","sequence":"first","affiliation":[{"name":"Department of Biology and Microbiology Universidad de Boyac\u00e1  Tunja Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5687-7077","authenticated-orcid":false,"given":"Adriana Marcela Valero","family":"Posada","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Universidad Santo Tom\u00e1s  Tunja Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3522-0818","authenticated-orcid":false,"given":"Martha Elizabeth Benavidez","family":"Rozo","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Universidad Santo Tom\u00e1s  Tunja Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0451-2535","authenticated-orcid":false,"given":"Mayra Eleonora Beltr\u00e1n","family":"Pineda","sequence":"additional","affiliation":[{"name":"Department of Biology and Microbiology Universidad de Boyac\u00e1  Tunja 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