{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T14:01:16Z","timestamp":1784901676414,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T00:00:00Z","timestamp":1732060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Consumers require fermented milk that possesses constipation\u2010relieving functions. To cater to the \u2018natural and additive\u2010free\u2019 consumption habit, this study is dedicated to developing probiotic fermented milk with constipation\u2010relief effects. Previously, we isolated two <jats:italic>Lactobacillus<\/jats:italic> strains, <jats:italic>Lactobacillus<\/jats:italic> MWLp\u201012 and <jats:italic>Lactobacillus<\/jats:italic> MWLf\u20104, from breast milk. This study evaluated the efficacy of these strains and their fermented milk in alleviating constipation in a Balb\/c mouse model of constipation. The evaluation criteria included fecal water content, time for first black feces expulsion and propulsion rate of the small intestine. The mechanisms of constipation relief were investigated using gastrointestinal regulatory peptides, colonic tissue pathology, short\u2010chain fatty acid levels and gut microbiota analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Fecal water content, time for first black feces expulsion and small intestine propulsion rate indicated that both MWLp\u201012 and MWLf\u20104, as well as their fermented milk, could alleviate constipation in mice. Fermented milk exhibited superior effectiveness for relieving constipation compared to that of the strains alone. The results related to gastrointestinal regulatory peptides and short\u2010chain fatty acids suggest that the mechanisms of constipation relief by the strains and their fermented milk may involve increased levels of 5\u2010hydroxytryptamine and substance P in the mouse serum, higher concentrations of short\u2010chain fatty acids in the intestines and decreased vasoactive intestinal peptide levels in the serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>MWLp\u201012, MWLf\u20104 and fermented milk relieve constipation in mice. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14032","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T04:10:45Z","timestamp":1732075845000},"page":"2594-2606","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mechanism analysis of the differences in relieving constipation in a Balb\/c 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