{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:45:52Z","timestamp":1790012752966,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003825","name":"Magyar Tudom\u00e1nyos Akad\u00e9mia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011019","name":"Nemzeti Kutat\u00e1si Fejleszt\u00e9si \u00e9s Innov\u00e1ci\u00f3s Hivatal","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011019","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,3,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Over the past two decades, the global incidence of gout has markedly increased, affecting people worldwide. Considering the side effects of xanthine oxidase (XO) inhibitor drugs (e.g. allopurinol and febuxostat) used in the treatment of hyperuricemia and gout, the potential application of phytochemicals has been widely studied. In addition, XO also takes part in the elimination of certain drugs, including 6\u2010mercaptopurine. In the current explorative study, we aimed to examine the potential effects of tea catechins, resveratrol, silymarin flavonolignans and some of their conjugated metabolites on XO\u2010catalyzed xanthine and 6\u2010mercaptopurine oxidation, applying <jats:italic>in vitro<\/jats:italic> assays and modeling studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Catechins, resveratrol and resveratrol conjugates exerted no or only weak inhibitory effects on XO. Silybin A, silybin B and isosilybin A were weak, silychristin was a moderate, while 2,3\u2010dehydrosilychristin was a potent inhibitor of the enzyme. Sulfate metabolites of silybin A, silybin B and isosilybin A were considerably stronger inhibitors compared to the parent flavonolignans, and the sulfation of 2,3\u2010dehydrosilychristin slightly increased its inhibitory potency. Silychristin was the sole flavonolignan tested, where sulfate conjugation decreased its inhibitory effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>2,3\u2010Dehydrosilychristin seems to be a promising candidate for examining its <jats:italic>in vivo<\/jats:italic> antihyperuricemic effects, because both the parent compound and its sulfate conjugate are highly potent inhibitors of XO. \u00a9 2024 The Author(s). <jats:italic>Journal of the Science of Food and Agriculture<\/jats:italic> published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14045","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T04:45:49Z","timestamp":1732769149000},"page":"2765-2776","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of catechins, resveratrol, silymarin components and some of their conjugates on xanthine oxidase\u2010catalyzed xanthine and 6\u2010mercaptopurine oxidation"],"prefix":"10.1002","volume":"105","author":[{"given":"T\u00edmea","family":"Bencsik","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orsolya","family":"Bal\u00e1zs","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Central Clinical Pharmacy, Faculty of Pharmacy University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00f3bert G","family":"Vida","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Central Clinical Pharmacy, Faculty of Pharmacy University of P\u00e9cs  P\u00e9cs Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs Z","family":"Zsid\u00f3","sequence":"additional","affiliation":[{"name":"Pharmacoinformatics Unit, Department of Pharmacology and Pharmacotherapy, Medical School University of P\u00e9cs  P\u00e9cs Hungary"},{"name":"National Laboratory for Drug Research and Development  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csaba","family":"Het\u00e9nyi","sequence":"additional","affiliation":[{"name":"Pharmacoinformatics Unit, Department of Pharmacology and Pharmacotherapy, Medical School University of P\u00e9cs  P\u00e9cs Hungary"},{"name":"National Laboratory for Drug Research and Development  Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate\u0159ina","family":"Valentov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences  Prague Czech 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