{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T17:06:33Z","timestamp":1778519193945,"version":"3.51.4"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The present study aimed to comprehensively evaluate the anticancer, anti\u2010inflammatory, and antioxidant properties of <jats:italic>Globularia cordifolia<\/jats:italic> L. samples. The plant material was collected and extracted using the maceration method. Antioxidant activities were assessed through DPPH (i.e. 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl) and iron chelation assays, whereas phenolic and flavonoid contents were measured using spectrophotometric methods. Gas chromatography\u2010mass spectrometry analysis was performed to identify biologically active compounds. The cytotoxic effect on colon cancer cells was evaluated using MTT [i.e. 2,3\u2010bis\u2010(2\u2010methoxy\u20104\u2010nitro\u20105\u2010sulfophenyl)\u20102H\u2010tetrazolium\u20105\u2010carboxanilide] assays, and inflammatory cytokine levels were measured through an enzyme\u2010linked immunosorbent assay. Molecular docking studies investigated the interactions of bioactive compounds with the 4UYA protein, and ADME (i.e. absorption, distribution, metabolism, excretion and toxicity) modeling predicted their toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The study revealed that the extract exhibited strong antioxidant activity with a median 50% inhibitory concentration (IC<jats:sub>50<\/jats:sub>) value of 39.48\u2009\u00b1\u20092.15\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup> in the DPPH assay and an effective iron chelation capacity with an IC<jats:sub>50<\/jats:sub> value of 4.28\u2009\u00b1\u20090.19\u2009mg\u2009mL<jats:sup>\u22121<\/jats:sup>. The total phenolic content was 662.51\u2009\u00b1\u200920.95\u2009mg gallic acid equivalents\u2009g<jats:sup>\u22121<\/jats:sup>, and the flavonoid content was 114.99\u2009\u00b1\u20090.51\u2009mg quercetin equivalents g<jats:sup>\u22121<\/jats:sup>, demonstrating the extract's richness in phenolic and flavonoid compounds. Significant antiproliferative effects were observed in colon cancer cells at a dose of 250\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>. Inflammatory cytokine analysis indicated a reduction in pro\u2010inflammatory responses. Molecular docking studies showed strong binding affinities between the bioactive compounds and the 4UYA protein, suggesting the mechanisms of action.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The findings indicate that <jats:italic>G. cordifolia<\/jats:italic> extract exhibits strong antioxidant, anti\u2010inflammatory and antiproliferative properties, making it a promising biomolecular agent for colon cancer therapy. The high phenolic and flavonoid contents, combined with the bioactive compounds' interactions with key molecular targets, underscore its potential therapeutic applications. \u00a9 2024 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14100","type":"journal-article","created":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T00:51:31Z","timestamp":1735347091000},"page":"3382-3396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of <scp><i>Globularia cordifolia<\/i><\/scp> on colon cancer cells: antioxidant activity, inflammatory cytokine analysis and molecular docking studies"],"prefix":"10.1002","volume":"105","author":[{"given":"Emine Incilay","family":"Torunoglu","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Medical Biochemistry Necmettin Erbakan University  Konya Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erdi 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