{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T02:52:56Z","timestamp":1788922376763,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772000"],"award-info":[{"award-number":["31772000"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Immobilized enzyme possessing both high activity and good selectivity is important in practice. In this study, <jats:italic>Candida antarctica<\/jats:italic> lipase B (CALB) was immobilized onto the macroporous resin ADS\u201017 for triacylglycerol (TAG) synthesis through esterification of oleic acid and glycerol. The reaction conditions were optimized by single\u2010factor study and orthogonal test, and the reusability of the immobilized CALB (CALB@ADS\u201017) was evaluated. In addition, the mechanism of lipase immobilization was studied and the catalytic mechanism of CALB@ADS\u201017 was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Oleic acid conversion up to 99.20% and TAG content at 91.58\u2009wt% could be obtained under optimal conditions. In addition, the CALB@ADS\u201017 retained 84.28% of its initial activity after 11\u2009cycles of reuse. The mechanism of lipase immobilization was through hydrophobic adsorption. The relationship between temperature and oleic acid conversion was ln<jats:italic>V<\/jats:italic><jats:sub>0<\/jats:sub>\u2009=\u20096.3316\u2009\u2212\u20094.3321\/<jats:italic>T<\/jats:italic>, and the activation energy (<jats:italic>E<\/jats:italic><jats:sub>a<\/jats:sub>) was 36.02\u2009kJ\u2009mol<jats:sup>\u22121<\/jats:sup>. CALB@ADS\u201017 did not exhibit an obvious interfacial activation phenomenon. Its kinetic behavior can be described by the Michaelis\u2013Menten model, whose kinetic parameters of <jats:italic>v<\/jats:italic><jats:sub>max<\/jats:sub>, <jats:italic>k<\/jats:italic><jats:sub>cat<\/jats:sub>, <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>, <jats:italic>K<\/jats:italic><jats:sub><jats:italic>i<\/jats:italic><\/jats:sub>, and <jats:italic>k<\/jats:italic><jats:sub>ca<jats:italic>t<\/jats:italic><\/jats:sub><jats:italic>\/K<\/jats:italic><jats:sub>m<\/jats:sub> were 0.01265\u2009\u03bcmol\u2009L<jats:sup>\u22121<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup>, 9310.72\u2009s<jats:sup>\u22121<\/jats:sup>, 0.4907\u2009mmol\u2009L<jats:sup>\u22121<\/jats:sup>, 3.997\u2009mmol\u2009L<jats:sup>\u22121<\/jats:sup>, and 1.90\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>\u2009L\u2009mmol<jats:sup>\u22121<\/jats:sup>\u2009s<jats:sup>\u22121<\/jats:sup>, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>CALB@ADS\u201017 showed good esterification performance and exhibited good selectivity towards TAG generation. In addition, CALB@ADS\u201017 exhibited good reusability in esterification reactions and has potential in practical applications. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14137","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T05:28:08Z","timestamp":1737437288000},"page":"3931-3941","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Selective synthesis of triacylglycerols by the <scp>ADS<\/scp>\u201017\u2010supported <i>Candida antarctica<\/i> lipase B through esterification of oleic acid and glycerol"],"prefix":"10.1002","volume":"105","author":[{"given":"Jiawei","family":"Zheng","sequence":"first","affiliation":[{"name":"School of Food Science Guangdong Pharmaceutical University  Zhongshan 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