{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:46:12Z","timestamp":1785419172011,"version":"3.56.0"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T00:00:00Z","timestamp":1743379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Hyperlipidemia is characterized by abnormally elevated blood lipids. Persimmon leaf has multiple pharmacological activities and is valued for its lipid\u2010lowering effect. However, few reports have revealed the hypolipidemic mechanism of persimmon leaf. In this study, compositions from the ethanol extract of persimmon leaf (PLE) were identified by high\u2010resolution liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry and high\u2010performance liquid chromatography. The mechanism of PLE against hyperlipidemia induced by high\u2010fat diet in mice was then explored based on lipid gene expression and gut microbiota.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The study demonstrated that 27 compositions from PLE were identified, of which the primary hypolipidemic compositions were astragalin, hyperoside, catechin, chlorogenic acid, and quercetin. Supplementation of PLE could reduce serum lipids, liver injury, lipid accumulation, and inflammation. The analysis of lipid gene expression indicated that PLE downregulated the expression of lipid synthesis genes FAS (fatty acid synthase; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), ACC (acetyl coenzyme A carboxylase; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), SCD1 (stearyl coenzyme A dehydrogenase 1; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and SREBP\u20101c (sterol regulatory element binding protein 1c; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), while upregulating the expression of lipid degradation genes PPAR\u2010\u03b1 (peroxide\u2010activated receptor alpha; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and CYP7A1 (cholesterol 7\u03b1\u2010hydroxylase; <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Simultaneously, PLE greatly recovered the intestinal short\u2010chain fatty acid content, especially butyric acid (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), valeric acid (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) and isovaleric acid (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Furthermore, 16S rRNA analysis showed that PLE decreased the Firmicutes\/Bacteroidetes ratio and increased the abundance of <jats:italic>Lactobacillus<\/jats:italic>, <jats:italic>Turicibacter<\/jats:italic>, and <jats:italic>Dubosiella<\/jats:italic> microbiota, which maintained the homeostasis of intestinal flora.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>PLE could prevent lipid metabolism disorders and modulate gut microbiota homeostasis in hyperlipidemic rats. This study provides insights into PLE as a natural active substance for the prevention of hyperlipidemia. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14248","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T22:41:30Z","timestamp":1743547290000},"page":"5074-5089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Persimmon leaf extract ameliorates hyperlipidemia by modulating lipid genes expression and gut microbiota in high\u2010fat\u2010diet\u2010fed mice"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6715-1430","authenticated-orcid":false,"given":"Yuanyuan","family":"Gao","sequence":"first","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuexin","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"He","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northwest A&amp;F University  Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuchang","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Food Science and Engineering Northwest A&amp;F University  Yangling People's Republic of China"},{"name":"Fuping Modern Agriculture Comprehensive Demonstration Station Northwest A&amp;F University  Fuping People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.125725"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2019.103694"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08391"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/88.4.894"},{"key":"e_1_2_7_6_1","first-page":"61","article-title":"What is the role of gut microbiota in obesity prevalence? 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