{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T03:48:02Z","timestamp":1776138482527,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Diet plays a significant role in mitigating inflammation, with lettuce and its polyphenols showing potential for improving inflammatory conditions. However, research on the material basis and mechanisms of action remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>This study investigated the primary components and anti\u2010inflammatory effects of the new lettuce cultivar Binfen\u20101, which is rich in polyphenols and may offer enhanced health benefits. There were 65 metabolites with significant changes in Binfen\u20101 by metabolomics, comprising mainly polyphenols. Meanwhile, the high\u2010polyphenol lettuce extract (HPLE) from Binfen\u20101 demonstrated superior antioxidant and anti\u2010inflammatory effects <jats:italic>in vitro<\/jats:italic>. Moreover, HPLE showed a significant improvement in mice with dextran sodium sulfate\u2010induced ulcerative colitis. By integrating network pharmacology with high\u2010performance liquid chromatographic quantitative results, isoquercitrin and chlorogenic acid were identified as the core active substances in HPLE, and the PI3K\u2010Akt, MAPK, and JAK\u2013STAT pathways were the key anti\u2010inflammatory pathways for HPLE, supported by mRNA validation and molecular docking predictions.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The core anti\u2010inflammatory substances in HPLE were isoquercitrin and chlorogenic acid, with the key anti\u2010inflammatory pathways being the PI3K\u2010Akt, MAPK, and JAK\u2013STAT pathways. These findings substantially propel the understanding of the therapeutic potential of lettuce in the treatment of inflammatory diseases. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14270","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T06:20:03Z","timestamp":1745907603000},"page":"5419-5432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolomics and network pharmacology reveal the anti\u2010inflammatory core substances and mechanisms of a new cultivar of high\u2010polyphenol lettuce"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3593-883X","authenticated-orcid":false,"given":"Qianshan","family":"Lu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering School of Biotechnology, East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxuan","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering School of Biotechnology, East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanzhe","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering School of Biotechnology, East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zitian","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering School of Biotechnology, East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Cao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioreactor Engineering School of Biotechnology, East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Zhejiang Jiameishan Plant Technology Co.  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