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One promising yet underexplored approach involves utilizing bacteria that produce volatile organic compounds (<jats:styled-content style=\"fixed-case\">VOCs<\/jats:styled-content>) with antifungal properties.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In this study, <jats:italic>Bacillus velezensis<\/jats:italic> <jats:styled-content style=\"fixed-case\">BF01<\/jats:styled-content>, a strain with significant antifungal activity, was identified and isolated. Volatile organic compounds produced by this strain inhibited the growth and conidial germination of <jats:italic>Botrytis cinerea<\/jats:italic>, <jats:italic>Fusarium oxysporum<\/jats:italic>, and <jats:italic>Colletotrichum gloeosporioides<\/jats:italic>. Gas chromatography\u2010mass spectrometry identified 90 <jats:styled-content style=\"fixed-case\">VOCs<\/jats:styled-content>, with ketones, esters, nitrogen compounds, aldehydes, and alcohols as the dominant components, many of which exhibited antifungal or plant resistance\u2010inducing properties. To enhance practical application, <jats:italic>B. velezensis<\/jats:italic> <jats:styled-content style=\"fixed-case\">BF01<\/jats:styled-content> was incorporated into a kaolin\u2010based composite, which maintained long\u2010term viability under refrigerated conditions and inhibited fungal growth through the controlled release of volatile organic compounds. This composite significantly extended the shelf life of strawberries by reducing postharvest decay.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The development of a kaolin\u2010based <jats:italic>B. velezensis<\/jats:italic> <jats:styled-content style=\"fixed-case\">BF01<\/jats:styled-content> composite provides an effective and sustainable biocontrol strategy for postharvest disease management. By improving bacterial stability and facilitating the controlled release of antifungal volatile organic compounds, this approach offers a practical alternative to chemical fungicides, highlighting its potential in extending fruit shelf life and improving food safety. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14275","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T09:42:57Z","timestamp":1744105377000},"page":"5507-5515","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Development of a kaolin\u2010based <scp><i>Bacillus velezensis<\/i> BF01<\/scp> composite for application in biocontrol of postharvest strawberry diseases"],"prefix":"10.1002","volume":"105","author":[{"given":"Hongling","family":"Lu","sequence":"first","affiliation":[{"name":"National Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya 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