{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T21:53:04Z","timestamp":1782769984982,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302071"],"award-info":[{"award-number":["32302071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272310"],"award-info":[{"award-number":["32272310"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2024GXNSFBA010260"],"award-info":[{"award-number":["2024GXNSFBA010260"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The inhibition of pancreatic lipase activity to reduce dietary lipid absorption is an effective strategy to combat obesity. Mangiferin, a bioactive flavonoid derived from <jats:italic>Mangifera indica<\/jats:italic>, exhibits potent lipid\u2010lowering potential. However, although its <jats:italic>in vitro<\/jats:italic> inhibitory effect on pancreatic lipase has been identified, the underlying mechanism remains poorly understood. Direct <jats:italic>in vivo<\/jats:italic> evidence of its impact on dietary lipid absorption is also lacking. This study aimed to elucidate the mechanism of mangiferin's inhibition of pancreatic lipase through <jats:italic>in vitro<\/jats:italic> inhibitory kinetics assays and <jats:italic>in silico<\/jats:italic> molecular docking analysis. Its effects on dietary lipid absorption were also evaluated in a mouse model of high\u2010fat diet (HFD)\u2010induced obesity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Inhibitory kinetics assays demonstrated that mangiferin inhibited pancreatic lipase in a reversible, non\u2010competitive manner, with a half\u2010 maximal inhibitory concentration (IC<jats:sub>50<\/jats:sub>) of 82.31\u2009\u03bcmol\u2009L<jats:sup>\u22121<\/jats:sup>. Molecular docking showed that mangiferin bound to the enzyme's allosteric site through hydrogen bonds with Glu\u2010188, Arg\u2010191, Gln\u201022, and Val\u201021 residues, consistent with non\u2010competitive inhibition. <jats:italic>In vivo<\/jats:italic> studies revealed that administration of 200\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> body weight of mangiferin alleviated HFD\u2010induced obesity, hepatic steatosis, and hyperlipidemia significantly. Notably, mangiferin increased fecal lipid excretion by 52.5%, which was significant, indicating reduced dietary lipid absorption.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that mangiferin is a promising pancreatic lipase inhibitor, which may reduce dietary lipid absorption, contributing, at least partially, to its lipid\u2010lowering effects. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14301","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T07:49:31Z","timestamp":1745394571000},"page":"5932-5939","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Lipid\u2010lowering effect of mangiferin through the inhibition of pancreatic lipase activity"],"prefix":"10.1002","volume":"105","author":[{"given":"Shuang","family":"Wang","sequence":"first","affiliation":[{"name":"School of Public Health Guangxi Medical University  Nanning China"},{"name":"Center for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Qin","sequence":"additional","affiliation":[{"name":"School of Public Health Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yashi","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Food Science and Engineering, Institute of Food Safety and Nutrition Jinan University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Public Health Guangxi Medical University  Nanning China"},{"name":"Center for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongxiang","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Public Health Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6545-2719","authenticated-orcid":false,"given":"Zouyan","family":"He","sequence":"additional","affiliation":[{"name":"School of Public Health Guangxi Medical University  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,23]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"LobsteinT Jackson\u2010LeachR PowisJ BrinsdenH GrayMandWorld Obesity Federation World Obesity Atlas 2023(2023). Available:https:\/\/www.worldobesity.org\/resources\/resource-library\/world-obesity-atlas-2023[5 April 2025]."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.15238"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0250-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110314"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200831010-00005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1182937"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10344"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/db14-0006"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315329"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102498"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3FO02791K"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201100392"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1983767"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2009.07.021"},{"key":"e_1_2_8_16_1","first-page":"21","article-title":"Inhibition of lipid metabolic enzymes using Mangifera indica extracts","volume":"4","author":"Moreno DA","year":"2006","journal-title":"J Food Agric Environ"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.5539\/jps.v5n2p72"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109798"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42832-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2022.844195"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12103053"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2021.101248"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026617666170130110827"},{"key":"e_1_2_8_24_1","first-page":"5","article-title":"Cobdock: an accurate and practical machine learning\u2010based consensus blind docking method","volume":"16","author":"Ugurlu SY","year":"2024","journal-title":"J Chem"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12986-020-00535-y"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2024.106466"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.13297"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1370143"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.08.052"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2020.104041"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2022.100424"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.7897\/2230-8407.1206145"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03245829"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.12774"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2015.05.017"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2010.00972.x"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.08.013"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jchemed.0c01184"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030782"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4MB00421C"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/bbb\/zbaa121"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.28.1672"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578x20910286"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.14301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:39:36Z","timestamp":1760884776000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.14301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,23]]},"references-count":42,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,8,30]]}},"alternative-id":["10.1002\/jsfa.14301"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.14301","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,23]]},"assertion":[{"value":"2025-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}