{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T13:01:04Z","timestamp":1771074064800,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T00:00:00Z","timestamp":1747267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100016692","name":"Key Research and Development Program of Ningxia","doi-asserted-by":"publisher","award":["2021BEG02027"],"award-info":[{"award-number":["2021BEG02027"]}],"id":[{"id":"10.13039\/100016692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>There are more and more Alzheimer's patients. The formation of plaques of A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub> in the brain is one of the main causes of Alzheimer's disease. Corn oligopeptides have natural antioxidant effects. It is aimed to develop functional corn oligopeptides to prevent Alzheimer's disease through antioxidation.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>According to previous laboratory studies, peptides of corn oligopeptides were screened by biological activity score and ADMET prediction, and molecular docking technology was used to screen the peptides that had high binding energy with A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub>. The protective effects of the selected peptides were evaluated against oxidative stress in A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub>\u2010injured SHSY5Y cells, and the mechanism of the effects in the protein kinase A (PKA)\/cAMP\u2010response element binding protein (CREB)\/brain\u2010derived neurotrophic factor (BDNF)\u2010mediated signaling pathway was investigated, which is closely related to neurodegenerative and neurological diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The results showed that three peptides (GL, FA and FQ) significantly increased the cell viability of A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub>\u2010induced cells and mitochondrial intensity and decreased extracellular lactate dehydrogenase content. They also improved intracellular oxidative stress caused by A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub>, including reducing the overproduction of intracellular reactive oxygen species, and increasing the content of lipid oxidation, superoxide dismutase and glutathione peroxidase. In addition, western blot showed that treatment with GL, FA and FQ significantly increased the expression of PKA, CREB and BDNF, whereas cells injured with A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub> decreased the expression of these signaling proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results suggest that peptides of corn oligopeptides can effectively improve A<jats:italic>\u03b2<\/jats:italic><jats:sub>1\u201042<\/jats:sub>\u2010induced Alzheimer's disease, and may improve oxidative stress response to protective nerve cells by up\u2010regulating the protein expression of the PKA\/CREB\/BDNF signaling pathway. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14307","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T02:45:49Z","timestamp":1747363549000},"page":"5738-5750","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Peptides of corn oligopeptides improve A<i>\u03b2<\/i><sub>1\u201042<\/sub>\u2010injured <scp>SHSY5Y<\/scp> cells"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6099-7145","authenticated-orcid":false,"given":"Hanshuo","family":"Wu","sequence":"first","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"},{"name":"College of Food and Biology Hebei University of Science and Technology  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hualei","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ganlu","family":"Meng","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8369-0809","authenticated-orcid":false,"given":"Wenying","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Protein &amp; Functional Peptides, China National Research Institute of Food and Fermentation Industries  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuguo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food and Biology Hebei University of Science and Technology  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"857","article-title":"Plasma amyloid\u2010\u03b2 (A\u03b242) correlates with cerebrospinal fluid A\u03b242 in Alzheimer's disease","volume":"62","author":"Teunnissen\u2010Charlotte E","year":"2018","journal-title":"J Alzheimers Dis"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27196751"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111575"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2015.01.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04983"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c06456"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9912436"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060890"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2015.11.128"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(19981115)19:14<>3.0.CO;2-B"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2449-y"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.0366-6999.20130224"},{"key":"e_1_2_7_14_1","first-page":"1019","article-title":"Advances in research on cAMP\/PKA\/CREB signaling pathway regulating tissue and organ cell fibrosis and intervention effect of traditional Chinese medicine","volume":"37","author":"Wei LX","year":"2020","journal-title":"Chin J Mod Appl Pharm"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42099-9"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.02.012"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-021-00865-7"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026618666180821145215"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CP01913A"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13311-016-0489-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2019.146322"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567205013666151218132725"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2023.105501"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21010072"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.04.018"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.10.049"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567205013666151218132725"},{"key":"e_1_2_7_28_1","first-page":"74","article-title":"Differences in the content of protein and essential amino acids between different rice varieties","volume":"8","author":"Zhang ZY","year":"2016","journal-title":"Asian Agric Res"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694-018-9833-1"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2014.05.003"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.07.009"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2015.0193"},{"key":"e_1_2_7_33_1","article-title":"Asiatic acid improves mitochondrial function, activates antioxidant response in the mouse brain and improves cognitive function in beta\u2010amyloid overexpressing mice","volume":"2","author":"Varada S","year":"2024","journal-title":"BioRxiv\u2010Neuroscience"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12122329"},{"key":"e_1_2_7_35_1","article-title":"Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage","volume":"17","author":"Liu J","year":"2023","journal-title":"Food Chem"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/09540105.2015.1079597"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2007.12.039"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.02.119"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-4337.2004.tb00058.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2014.06.022"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2012.11.009"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2018.01.090"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.103707"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1024\/0300-9831\/a000543"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(00)00422-8"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2014.05.014"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf502412f"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2012.01.050"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750-3841.13953"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12051519"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.3892\/MMR.2020.11548"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2020.02.027"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2009.04.120"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2011.19.2.224"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/trc2.12050"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.14307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:38:21Z","timestamp":1760884701000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.14307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,15]]},"references-count":54,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,8,30]]}},"alternative-id":["10.1002\/jsfa.14307"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.14307","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,15]]},"assertion":[{"value":"2024-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}