{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:57:34Z","timestamp":1787237854999,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472369"],"award-info":[{"award-number":["32472369"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005706","name":"Beijing Technology and Business University","doi-asserted-by":"publisher","award":["19008022334"],"award-info":[{"award-number":["19008022334"]}],"id":[{"id":"10.13039\/501100005706","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>This study aims to prepare collagen peptides from bovine bone meal using a combination of heat pretreatment and enzymatic digestion, and to chelate them with calcium chloride to form peptide\u2013calcium chelates. The effects of both on the proliferation and differentiation of osteoclasts were investigated using cellular experiments (RAW 264.7 cells).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Both bovine collagen peptides and their calcium chelates (BPs, HBPs, BPs\u2013Ca, and HBPs\u2013Ca) can significantly inhibit the RANKL\u2010induced differentiation of RAW 264.7 cells into osteoclasts. The preheating treatment before enzymatic hydrolysis of bone materials has an improving effect on the inhibition of RAW 264.7 differentiation into osteoblasts by collagen peptides and their peptide calcium chelates. HBP and HBPs\u2013Ca could significantly activate the NF\u2010\u03baB signaling pathway, among which HBPs\u2013Ca was the most effective, which could significantly downregulate the mRNA expression of genes related to osteoclast differentiation, such as AP\u20101, c\u2010Fos, TRAP, and NFATc1. Additionally, the expression of NF\u2010\u03baB p65, c\u2010Fos, IKK and I\u03baB\u03b1 were also significantly inhibited after treatment with HBPs\u2013Ca, with IKK being the most significantly downregulated, with an 8.2\u2010fold reduction compared to the control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>HBPs and HBPs\u2013Ca demonstrated stronger activity in inhibiting osteoclast formation compared to BPs and BPs\u2013Ca. This enhanced activity is likely due to structural changes in the peptides caused by heat treatment, which increase their antioxidant properties and antagonistic effects on RANKL. These findings indicate that bovine collagen peptides and their calcium chelates can inhibit the formation of osteoclasts by activating the NF\u2010\u03baB pathway, thereby influencing bone metabolism and providing a theoretical basis for the treatment of osteoporosis. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.14308","type":"journal-article","created":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T23:30:48Z","timestamp":1746142248000},"page":"4999-5008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bovine collagen peptides and peptide\u2013calcium complexes inhibit\n                    <scp>RANKL<\/scp>\n                    \u2010induced osteoclast differentiation in\n                    <scp>RAW<\/scp>\n                    264.7 macrophages"],"prefix":"10.1002","volume":"105","author":[{"given":"Yuanyi","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiying","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guofeng","family":"Jin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education School of Food and Health, Beijing of Technology and Business University  Beijing 100048 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.3258"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-019-00507-z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2012.05.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3275\/8522"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092073"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/814057"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00507"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2006.09.023"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-366-6:153"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.03.018"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1514"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2019.04.007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2011.02455.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2021.100901"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2019.4269"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.202200726"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11182762"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.26599\/FSHW2022.9250166"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0277871"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e25601"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/md17070404"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2019.103701"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology10020117"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2017.09.015"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fshw.2022.06.022"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051150"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205063200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00332.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2015.11.015"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01757-9"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.32.20551"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods13172778"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2020.110209"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2012.03.017"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.11.088"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.05.008"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.04.148"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.5940"}],"container-title":["Journal of the Science of Food and Agriculture"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jsfa.14308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:25:06Z","timestamp":1760883906000},"score":1,"resource":{"primary":{"URL":"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/10.1002\/jsfa.14308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/jsfa.14308"],"URL":"https:\/\/doi.org\/10.1002\/jsfa.14308","archive":["Portico"],"relation":{},"ISSN":["0022-5142","1097-0010"],"issn-type":[{"value":"0022-5142","type":"print"},{"value":"1097-0010","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-10-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}