{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T05:37:22Z","timestamp":1781069842009,"version":"3.54.1"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011231","name":"State Key Laboratory of Food Science and Technology","doi-asserted-by":"publisher","award":["SKLF\u2010ZZB\u2010202125"],"award-info":[{"award-number":["SKLF\u2010ZZB\u2010202125"]}],"id":[{"id":"10.13039\/501100011231","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Phenolic acids, such as chlorogenic acid (CGA) and rosmarinic acid (RA), are added to plant\u2010based beverages as nutritional supplements to enhance their health benefits. However, these compounds can also interfere with the astringency\u2010reducing effect of tannase. This study employed electronic tongue analysis, enzyme inhibition kinetics, spectroscopy, molecular docking and molecular dynamics simulations to investigate the inhibitory mechanisms of CGA and RA on tannase\u2010mediated deastringency.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Our research results indicate that CGA can inhibit tannase\u2010mediated deastringency. It, along with RA, inhibits tannase activity in a non\u2010competitive manner and quenches its intrinsic fluorescence through static quenching. The binding of CGA and RA to tannase led to the exposure of aromatic amino acid residues and a more polar microenvironment. Fourier transform infrared spectroscopy showed that CGA and RA reduced the <jats:italic>\u03b1<\/jats:italic>\u2010helix and <jats:italic>\u03b2<\/jats:italic>\u2010turn content in tannase, while increasing the unordered coil content. Molecular docking and dynamics simulations revealed that CGA and RA bind tightly to tannase primarily through hydrogen bonds and van der Waals interactions, occupying the substrate\u2010binding site and thus inhibiting tannase's astringency\u2010reducing activity. Additionally, other polyphenols, such as epicatechin, hesperidin and naringin, were also found to inhibit tannase activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The study demonstrated that CGA and RA inhibit the astringency\u2010removal activity of tannase, offering important mechanistic insights for the development of plant\u2010based beverages and deastringency techniques. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14314","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T01:12:54Z","timestamp":1745975574000},"page":"6094-6105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Inhibitory effect of chlorogenic acid on tannase\u2010mediated astringency removal and its mechanism"],"prefix":"10.1002","volume":"105","author":[{"given":"Qianyu","family":"Shen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Institute of Nutrition Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"You","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Institute of Nutrition Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhike","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Resources, Institute of Nutrition Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangxi Runzhi Agriculture Co. 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