{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:29:16Z","timestamp":1787725756491,"version":"build-2784847793"},"reference-count":77,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32001690"],"award-info":[{"award-number":["32001690"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023QC145"],"award-info":[{"award-number":["ZR2023QC145"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Naringin, a natural flavonoid compound, has demonstrated potential anti\u2010obesity effects by modulating lipid metabolism. Pancreatic lipase plays a critical role in lipid digestion and absorption, making it a key target for obesity management. This study investigated the inhibitory mechanism of naringin on pancreatic lipase through\n                      <jats:italic>in vitro<\/jats:italic>\n                      ,\n                      <jats:italic>in vivo<\/jats:italic>\n                      , and\n                      <jats:italic>in silico<\/jats:italic>\n                      experiments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      <jats:italic>In vitro<\/jats:italic>\n                      experiments revealed that naringin was a competitive inhibitor of pancreatic lipase, with a half\u2010maximal inhibitory concentration (IC\u2085\u2080) of 6.93\u2009\u00b1\u20090.12\u2009mmol\/L. Multi\u2010fluorescence spectroscopy confirmed that naringin induced secondary structure changes and static fluorescence quenching of pancreatic lipase, with a binding constant of 2.16\u2009\n                      <jats:bold>\u00d7<\/jats:bold>\n                      \u200910\n                      <jats:sup>4<\/jats:sup>\n                      \u2009L\/mol. Isothermal titration calorimetry indicated a spontaneous exothermic reaction (\u221226.08\u2009kJ\/mol), mainly driven by hydrogen bonding and electrostatic force.\n                      <jats:italic>In vivo<\/jats:italic>\n                      studies demonstrated that naringin decreased serum lipase activity by 24.54%, mitigated hepatic lipid accumulation, and down\u2010regulated inflammatory cytokines in high\u2010fat diet\u2010induced obese rats. Molecular simulation further revealed that hydrogen bonding was the primary interaction force between naringin and pancreatic lipase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>Naringin effectively inhibits pancreatic lipase through competitive binding and structural modifications, reducing lipid digestion and absorption. The inhibitory effect of naringin on pancreatic lipase might provide new insights into the role of naringin in lipid metabolism regulation and potential application in obesity management. \u00a9 2025 Society of Chemical Industry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jsfa.14336","type":"journal-article","created":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T04:59:09Z","timestamp":1746593949000},"page":"6229-6242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular mechanisms of naringin's high lipid\u2010lowering activity: pancreatic lipase inhibition and fat accumulation reduction"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7439-7587","authenticated-orcid":false,"given":"Kai","family":"Yu","sequence":"first","affiliation":[{"name":"Orthopedics Department China Aerospace Science and Industry Corporation 731 Hospital  Beijing 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