{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T23:47:24Z","timestamp":1793490444921,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Metabolic\u2010associated fatty liver disease (MAFLD) is a chronic liver condition characterized by hepatic fat accumulation and metabolic abnormalities. Its pathology is closely linked to lipid metabolism disorders, chronic inflammation, and oxidative stress. Dietary supplementation has recently emerged as a promising treatment strategy for MAFLD. Broccoli leaves, a by\u2010product of broccoli, are rich in proteins, polysaccharides, polyphenols, flavonoids, and other bioactive compounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This study identified the active components in broccoli leaf crude extract (BLCE) using high\u2010performance liquid chromatography with tandem mass spectrometry (HPLC\u2010MS\/MS) and chemical methods, and confirmed its significant antioxidant activity through three <jats:italic>in vitro<\/jats:italic> assays. In oleic acid (OA)\u2010induced HepG2 cells, BLCE attenuated the overproduction of reactive oxygen species (ROS), restored antioxidant markers (SOD, MDA, and GSH), and reduced intracellular total cholesterol (TC) and triglycerides (TG). In lipopolysaccharide (LPS)\u2010induced RAW264.7 cells, BLCE significantly inhibited the production of inflammatory factors \u2013 tumor necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic> (TNF\u2010<jats:italic>\u03b1<\/jats:italic>), interleukin\u20106 (IL\u20106), and interleukin\u20101<jats:italic>\u03b2<\/jats:italic> (IL\u20101<jats:italic>\u03b2<\/jats:italic>) \u2013 demonstrating anti\u2010inflammatory effects. In a high\u2010fat diet (HFD)\u2010induced MAFLD mouse model, BLCE reversed MAFLD by lowering serum and liver lipids, restoring liver function, morphology, and organization, and reducing liver injury, inflammation, and oxidative stress. Finally, the study identified 487 intersecting genes and screened 20 core targets through network pharmacology. Enrichment analysis revealed that BLCE's anti\u2010MAFLD effects mainly involve hormone regulation, the carboxylic acid metabolic process, and the cAMP signaling pathway. Moreover, molecular docking results showed that glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH), a central target, and key compounds in BLCE exhibit significant affinity and bind effectively, contributing to anti\u2010MAFLD effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Broccoli leaf crude extract may be an effective dietary supplement for alleviating MAFLD by suppressing hepatic lipid accumulation, oxidative stress, and inflammation. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14423","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T04:08:31Z","timestamp":1754539711000},"page":"7210-7226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Broccoli leaf crude extract: a promising remedy for metabolic associated fatty liver diseases through lipid reduction, antioxidation, and 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