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Esterified butyric and valeric acids (EBVA) support intestinal integrity, but their combined effects on broiler performance and gut health remain underexplored. This research evaluated EBVA supplementation on broiler growth and intestinal health. Chicks were assigned to two groups: a positive control receiving the basal diet and an EBVA group receiving the basal diet supplemented with 500\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> EBVA from day 1 to 28 and 250\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> from day 29 to 42.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>EBVA significantly improved average daily gain and feed conversion ratio during days 29\u201342 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and 1\u201342 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). EBVA significantly improved villus height in the jejunum and ileum (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and increased jejunum villus\u2010to\u2010crypt ratio at day 42 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The mRNA expressions of <jats:italic>Occludin<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and <jats:italic>Claudin\u20101<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) were significantly higher in the EBVA jejunal mucosa at day 28, while <jats:italic>Zonula occludens\u20101<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and <jats:italic>Occludin<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) showed increased expression at day 42. EBVA modified the microbiota, significantly increasing <jats:italic>Bacillus<\/jats:italic> spp. in the terminal ileum at day 14 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) and Eggerthellaceae spp. at day 42 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), while reducing <jats:italic>Streptococcus<\/jats:italic> spp. at day 28 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>EBVA supplementation improved broiler growth and intestinal health by improving intestinal morphology, increasing barrier protein expression and modifying microbiota, suggesting potential for improved poultry production. \u00a9 2025 Society of Chemical Industry.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jsfa.14441","type":"journal-article","created":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T23:25:49Z","timestamp":1751671549000},"page":"7293-7309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Esterified butyric and valeric acids enhance broiler growth and intestinal health by modulating gut morphology and microbial profile"],"prefix":"10.1002","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6291-3935","authenticated-orcid":false,"given":"Changchun","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of Agriculture Feed Research Institute, Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Feed Biotechnology of Ministry of 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