{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:48:08Z","timestamp":1785181688676,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T00:00:00Z","timestamp":1277769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["scijournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Sci Food Agric"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> Lincomycin (LIN) is an antibiotic widely used in veterinary medicine to cure infections caused by Gram\u2010positive pathogens. Although the toxicity of LIN is not serious, it will cause adverse effects in humans, such as pseudomembranous enteritis and bacterial resistance. In this study, for the preparation of a LIN derivative, a novel modification method was adopted. The LIN derivative modified at 2\u2010position with a carboxylic group at the end of the spacer was synthesised and coupled to carrier proteins. A LIN polyclonal antibody\u2010based enzyme\u2010linked immunosorbent assay (ELISA) was developed and characterised.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> The ELISA standard curve was constructed with concentrations of 0.1\u20131000 ng mL<jats:sup>\u22121<\/jats:sup>. The IC<jats:sub>50<\/jats:sub> value for nine standard curves was in the range 23.7\u201329.3 ng mL<jats:sup>\u22121<\/jats:sup> and the limit of detection at a signal\u2010to\u2010noise ratio of 3 was 0.15\u20130.98 ng mL<jats:sup>\u22121<\/jats:sup>. The cross\u2010reactivity value of the LIN antibody with clindamycin hydrochloride, a homologue of LIN with similar molecular structure, was 18.9%, while less than 0.1% cross\u2010reactivity was found with seven other compounds. For LIN\u2010spiked food samples, the recoveries and relative standard deviation (RSD) were 76.6\u2013117.6% and 1.7\u201334.6%, respectively.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> The proposed ELISA can be utilised as a sensitive and specific analytical tool for the detection of LIN in food samples. Copyright \u00a9 2010 Society of Chemical Industry<\/jats:p>","DOI":"10.1002\/jsfa.4055","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T11:53:33Z","timestamp":1277812413000},"page":"2083-2089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A sensitive and specific enzyme\u2010linked immunosorbent assay for the detection of lincomycin in food 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