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Some medicinal plants exert strong antifungal properties and could be conveniently used as a promising alternative source for presently problematic antifungal treatment in many areas with respect to their natural origin. Methanol extracts of 46 medicinal plants from the Eurasian area were used in a screening assay for antifungal activity in this study. The growth inhibitory effect was tested against six significant pathogenic and toxinogenic fungal species: <jats:italic>Fusarium oxysporum, F. verticillioides, Penicillium expansum, P. brevicompactum, Aspergillus flavus<\/jats:italic> and <jats:italic>A. fumigatus<\/jats:italic>.<\/jats:p><jats:p><jats:bold>RESULTS:<\/jats:bold> For 14 plant species, the possibility of using them as natural fungicides was indicated. The extract from <jats:italic>Grindelia camporum<\/jats:italic> showed significant activity against all target fungal species. The most sensitive target fungus was the toxinogenic and human pathogenic species <jats:italic>A. fumigatus<\/jats:italic>.<\/jats:p><jats:p><jats:bold>CONCLUSION:<\/jats:bold> This study has identified 14 extracts of medicinal plants with a potential use as an antifungal treatment in various areas. One of them showed promising efficiency against all selected significant pathogenic and toxinogenic fungal species. 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